FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1803_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422380
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC53010.11986758261388665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG9450.034.5242429
GCGAAAG13700.034.4032972
CGAAAGC16550.028.0407283
GTAACGC1855.8148216E-627.4368113
AGCGAAA17300.026.8250871
TATAATA33050.022.5981582
TATACTA18350.022.128872
GTATTAT49650.020.4463631
GAATAAC37850.019.5407661
GTCCTAT44300.019.3145921
TACTACA32400.018.7992954
GGTAGTG27650.018.6170837
ATACTAC32150.018.4943963
ATAATAC44850.018.1076833
TAGAGTG30050.018.0977295
TCCTATG44150.018.0663032
TAATACA52300.017.8852634
TAAACGT8350.017.368044
TTAGAGT28300.017.167054
TATACAT37550.016.9933915
Produced by FastQC (version 0.11.5)