FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1803_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGGCTTG30.2005347593582888No Hit
TGTCAAAGCATCTCTTGTCGGCCTGTACCCAGCAAGTAAGAAAATAACAT30.2005347593582888No Hit
GCATGAGGTCATGTGGTCCTCATGAGGCTGACCTACCCAAGGGGCTGGGG20.1336898395721925No Hit
GCCCTGAGGCCAGGTCTGGAGTGGCTGATGGCCTCGGCCAGTGTCCTCGG20.1336898395721925No Hit
GTTCGGGATGGTCTCGATCCTGTTGCTGTCCAGGTAGAGCTGGTGAATGG20.1336898395721925No Hit
CTATATAAGAATATTCCTGATGGAGACTCTTGGCTTTGACTTGAACAGGG20.1336898395721925No Hit
AAGTATTTCTGTGTTCCTATTTTGGTTTGCCCACGAACCCAATTGGTTGT20.1336898395721925No Hit
CAGTCCCCTGGGACCATGACCTGAGCAGAAGGCAGACACTTAACGACTGA20.1336898395721925No Hit
GTTTGGGTCAAGGACTGTTGTAATTCCACATTGTTTCCAATTCATTGTTC20.1336898395721925No Hit
GGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT20.1336898395721925No Hit
CTCCTGGAATCCTCCTCCTGTGAGGCCTCGTGTGGTTTGGGGCTGGCGCC20.1336898395721925No Hit
CTATAATAGTAGTTTACCTTGAATCAAGTGAAGATGGTAAAAAATATGCC20.1336898395721925No Hit
TAATAGAACAGACCATTTTCGTCTAATAGAACTTAAAGGGTGAGATGAAA20.1336898395721925No Hit
ATTCACAGACCTGTACCCCTGGGGCTAATAATACATTATATGTTTATTTA20.1336898395721925No Hit
TCCATGGGCAGGAGGTCGTCATTTGCATGGATCCAGAGGACCTACCGCCC20.1336898395721925No Hit
GAGGGAGAGGTGGTCCTCCTGCTCTTGTGCAGCCAAGACCTCTAATCTAT20.1336898395721925No Hit
ACCTTGTACTTGGTGGTAAGCATAAACATTCTCTTCAGGACAAGCACTTC20.1336898395721925No Hit
GTTATATTCAAAGTGATGAAAGAAAAAATTCCTCCCAACCAAGAATACTC20.1336898395721925No Hit
AATTTATACTGTAGATATTAACTACGAGAGCCCCAACAATGGTGGTTGGC20.1336898395721925No Hit
AGTTGATTGTATTTCTTGTTATCTGTGCTCTTTCTCTTCATAATTACAAA20.1336898395721925No Hit
GCCTGGCAGGGAGGGGCCCGAAGAACGGCCGAGATGGGGTCTGGCTGGTG20.1336898395721925No Hit
CGGTAGAGCCACTCCAGTCCCAGCGTTTGCCAGATTTTCGGTGCGCGTTT20.1336898395721925No Hit
TTCTTAAATCTAGGCAAGTAGAGTGGAATTATCATAAGAGGGTCAGACTA20.1336898395721925No Hit
GAGCAGAGCCCCACTTGGGGCTTGATCTCTTGACCCTGAAATCATGATCT20.1336898395721925No Hit
CCCTAAATGTTCCACACGTGATAATTCTAACTACCAGTCTAGAGTCCTGA20.1336898395721925No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT20.1336898395721925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)