FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1803_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262846
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17250.026.0617052
TATATCG1801.519836E-424.1701325
AGCGAAA18100.024.0365941
CGAAAGC20900.021.5102583
CCTACGG5103.274181E-1119.9048143
GTATATA21500.019.5609441
CTACGGT5305.820766E-1119.1536884
TACACCG8400.018.9908165
TAGGACT23700.018.6630124
TATACTG22100.017.389375
CGTTATA5602.4538167E-916.8327711
TATACAT23450.016.6974895
GTCCTAT29750.016.5738051
GTATTAT30900.015.2529951
CTAGGAC15800.015.1445763
GTAGCCG7704.7293724E-1115.0645559
TACGGTG7805.820766E-1114.8739285
CCCTACG8801.8189894E-1214.831672
CACGAGC9350.014.732248145
TTATAGT17400.014.5854234
Produced by FastQC (version 0.11.5)