FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1803_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4282230
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC3450.042.0337751
TATACTG26350.031.6449725
CTACGCA4850.029.9003142
GTGATCG6850.027.5188149
TAGCGCG2954.838512E-1027.033506145
GTAATAT20700.025.9208281
GCGAAAG19800.025.6342352
AGCGAAA20900.023.2442761
GTTACGT5200.022.3102361
CGAAAGC23400.021.6905043
ATACTGT35900.020.399266
GTTGACA32750.020.3687341
GTATTAT46550.019.782061
CAATTCG2256.8717706E-419.3337299
AGTCGAA2701.0792783E-418.7984372
ATTATAC16600.018.782263
CCGACTA8900.018.738094
GTCGAAG3652.9863913E-617.878753
TCGCACC2851.633171E-417.8090464
CGTAGAC5001.1059456E-817.4019813
Produced by FastQC (version 0.11.5)