FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1803_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192911
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19200.037.0115472
CGAAAGC23950.030.5793483
TATACTG42400.030.4418285
AGCGAAA23250.030.2527871
TACGCAC7300.029.7996233
CTACGCA7700.029.1933142
ACTACGC7600.028.6235981
TTATACT35800.022.2805654
GTATAGG17100.020.7786121
ATACCGA8050.019.8172516
GTAATAT28850.018.8508971
GTGATCG7700.018.831319
CGCACTG14700.018.7448865
TACACCG13600.017.5950955
TATACTA14950.017.4613515
ACGCACT13400.017.3165654
TATACCG9350.017.0619095
CCTTATA34500.017.0246542
ATACTGT46600.016.6500076
ATCGTGC6104.274625E-1016.639158
Produced by FastQC (version 0.11.5)