FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1803_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591980
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC55730.12136376900596256No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC604.946376E-448.3344739
GCGAAAG15600.037.18322
TATAGCG5100.036.9644742
ATAGCGG5600.033.6640743
AGCGAAA17500.032.317511
AGCGGTA6200.030.4059285
GCGGTAT6950.027.125016
CGAAAGC22700.025.5532133
CGGTATC7750.024.3226267
CTCCGAC5700.024.166183145
TATACTG34350.023.430035
TCTATAC51600.022.4828643
ATGCCGG17050.020.410149145
GTTCTAT55400.019.7628041
GTCCTAT48000.019.6373771
TAGCGGT10600.019.1526474
GTATAAG19700.018.4028511
TATACCA65200.018.3490245
CTATACC64500.018.3233364
GTATAGG16300.018.2380181
Produced by FastQC (version 0.11.5)