FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1803_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9299851
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC786870.8461103301547519No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG549420.5907836587919527No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA455610.48991107491937236No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG291690.3136501864384709No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG290870.3127684518816484No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT278560.2995316806688624No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA248710.2674343922284346No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC233110.2506599299279096No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG230780.2481545134432799No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC222120.2388425362943987No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA215930.23218651567643395No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT214270.23040154084189088No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT208500.22419714036278643No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC205400.22086375362357957No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC203260.2185626414874819No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG199930.21498193895794676No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC187240.20133655904809658No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA186170.20018600298004774No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA184500.19839027528505562No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT182530.1962719617765919No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC181210.1948525841973167No Hit
TTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTGT174890.18805677639351426No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT173790.18687396174411827No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG173040.1860674972104392No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC165530.17799209901319923No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT159880.17191673285948345No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG158050.16994895939730648No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT155650.1673682728895334No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC153040.16456177631233015No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC145730.15670143532407133No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC142420.1531422385154343No Hit
ATCTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTG139780.15030348335688387No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC132140.14208829797380623No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG127440.13703445356275062No Hit
CTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTA126650.13618497758727532No Hit
ATGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATT124560.1339376297534229No Hit
TGCTAGTACTACTTGAAAATCAAAAAACACTCGATGAGCATGATGCGAAC123430.13272255652267978No Hit
GCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGG119240.12821710799452593No Hit
GCAATGTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAA118410.1273246205772544No Hit
ACCTAGAGGAACTCAGGACACTTTTTAGTTCCGCTAGTTCCTACCAAAGA111380.1197653596815691No Hit
ATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAGACACA110560.11888362512474662No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA109850.11812017203286375No Hit
ACATTGCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTC109620.11787285624253549No Hit
GAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCT109170.11738897752232805No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG108710.11689434594167154No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG108470.11663627729089424No Hit
GCTGTAAATGGAACGTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACT107440.11552873266464161No Hit
CGCTAACAGAAACCAATGTTCCTGTGACACATGCCAAAGAATTGCTCCAC106540.1145609752242267No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT106070.11405559078312116No Hit
AGCCTGGGACATCCCCTCATTCTAGACACATGCACTATTGAAGGACTAGT105510.11345343059797408No Hit
GCCTGGGACATCCCCTCATTCTAGACACATGCACTATTGAAGGACTAGTC104350.11220609878588378No Hit
GTTATTTGTGTATTGGGCGTCTTGAACAGGGTAAAAACCGCTCTTTTGAG103980.11180824294926876No Hit
GGTTTGAAGGTCCTATTCAAATCTTCTGTTGTCACGCTTGTTGTTGTGTC103840.11165770290298199No Hit
TGTCAATGGTCTGCAGGGAAGAATTGATTATTATTGGTCGGTACTAAAAC103780.11159318574028768No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC103440.11122758848501982No Hit
TTCCAGTGTAAGTCACATTCCAGGTTGTGTCTGGGAAGATTTGGATTCTT103200.11096951983424251No Hit
AGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGCCACCAGTCAA103130.11089424981109912No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA101300.10892647634892215No Hit
CCATTGACAAGGGGCCTTGGCCCTATCACTGGTTTGAAGGTCCTATTCAA98500.10591567542318689No Hit
ATATTGTGGAATGGCAATGTACTGTTTAAGCCACCTTTTTCAGTCTGACA97810.10517372805220213No Hit
GTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGTT96970.10427048777448154No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG96890.10418446489088912No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT95820.10303390882284028No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC93590.10063602094270113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA33050.090.828482
GGTACTG35750.083.563515
CAGGTAC40900.073.395633
GTACTGA42600.072.679816
AGGTACT46850.065.467234
TATACTA37650.060.0873152
GACTTAA10550.059.09845145
GACAGTA44200.056.7551047
CGCAATT5050.054.553463145
TTTGGAC50750.051.43513
TAACTAT20500.050.933021
GGATAGC93450.050.201945
GCGAAAG10350.049.7406122
ACTATAC23450.047.617993
AACTATA23500.045.9739272
TATGGAT104600.045.3355872
ACTGATC127300.043.6252068
ATACTAC53450.043.4105643
GTATAGG278450.042.5496181
ATAGGTG280700.042.2346153
Produced by FastQC (version 0.11.5)