FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1803_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1803_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG11.5873015873015872No Hit
GACCCGGCTGTCCCATGAATATGGCTGCCCACCTCTGCCCTCGTAGACCG11.5873015873015872No Hit
GCCCCGACTTACCCTCGTTTCTGACTGTGTGCACCCCGCGGAGTATATTC11.5873015873015872No Hit
GGAAACAGCCTCTGAAAACAGAGATAACATCTCTTTTCAGAGCCAAGGGC11.5873015873015872No Hit
CCTGTGCCCGGGCACTCTCTCCGTGGGCATTTTAGGTGGGCAGATATAGA11.5873015873015872No Hit
GGACAATAGGTACTGAGCTCTTAGCGCAGACCAGGCTTTACCATGAATAT11.5873015873015872No Hit
GTCCAAACACTGTAGTCCTCATTAGTAGCTGTCCTCATAGTAGCTGTGTG11.5873015873015872No Hit
AGGAAGAGCATTCCAGGTGGAAAGAACAAGGTGAAAAGCAGACCAGTGAG11.5873015873015872No Hit
GCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGATCACACA11.5873015873015872No Hit
CTGCACCTTTCCACCCTCCCCGCACCTTTCCCCCCTCTTGGGGCTACCTG11.5873015873015872No Hit
GAGTTGAACCAAGATTCATTAAGCAAAACAAAGGTATCATTAAAAAGGAA11.5873015873015872No Hit
GCACAGAACCCAACGTGGGGTTTAATTTCACAGCCCTGAGATCATGACCT11.5873015873015872No Hit
TGATCCCGGGACCCAGGGATCATGACCTGAGCTGAAGGCAGCTGCGTAAC11.5873015873015872No Hit
GAGTGCCCGGGCACAGGGAGGGGGTTCATCACTGTTTCTTTTTCTAATTT11.5873015873015872No Hit
GAAGGGGGCTGGAGTCAGGAAGACCGGGTTGGCAATTCTGCTTCTGGGGT11.5873015873015872No Hit
GGAGCAGAACAAGAAAAAATCCCAACACTGTAACAAGAGCCAATGAAGGG11.5873015873015872No Hit
AGGGGACACTCTCGCAAAGAGGAAGGCCAGCATCTGTCCATGACAGGAGG11.5873015873015872No Hit
GGTTTAGGATGTGGCCCTGAGGCCAGGTCTGGAGTGGCTGATGGCCTCGG11.5873015873015872No Hit
GTATACTTAAGAATTTGTAGCAGTTCTTTCTTTTCTCAAATACTGGTCAA11.5873015873015872No Hit
GATATTTGTGTATAGGGGGTCGAGCCGATGGTACAAAACCCTGTGTTGAT11.5873015873015872No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTGCAGGGATGATGATGGGCAT11.5873015873015872No Hit
GCCCGCAAGCCCGGTCGCTTTGGGATGAGTCGGGAGGTCACCTGCCCGCA11.5873015873015872No Hit
GTTCAGGAAGGAGGGAATGGATCACATAGCACCGTGTTCACCCTTGAGAA11.5873015873015872No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC11.5873015873015872No Hit
GCTGTCAACAGAGCGCTGGGTGTGGGGGACATCAGTTCCAAACTGAGGAC11.5873015873015872No Hit
GAACAGGACACAGTCACTCGGCCCAACACTGGCCAGAGCTCCCATGAGTG11.5873015873015872No Hit
GTCCCTGAACATCTGCATTCGGCTATGGTCATCATCTCAGGGGCCTGGGA11.5873015873015872No Hit
GTATAGGTGGGTGGGTGATGTATGCACAACACGAAAAGAATGCTCTTTCC11.5873015873015872No Hit
GAGCCACAGAAAGGGCCAGATCACACCCTGTGGAACACAATGCGATGTTA11.5873015873015872No Hit
CCCATATAGCCATGGAACAGGAACAGGATAGCAGTTCATATCGGAGGCCA11.5873015873015872No Hit
ATGTCATGTCAAAATTCCCCCATCCATTTTCGTCAGCAACTCATTCCAAA11.5873015873015872No Hit
GGGCAGGAATGGGCACCAAGGGGCTCTGACAGGCTGCCCAGCCCCCCTCT11.5873015873015872No Hit
GCAGAGGGCAAGGCCTGGGTCGGCTTCCAGTCCTGCTTTCAAGCCTGCTT11.5873015873015872No Hit
GGTTGCTTATTAAGTTAGGTTGCTTATTTGAGATGTTTCTTGTTTCTTGA11.5873015873015872No Hit
GATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACAGAGTCTCA11.5873015873015872No Hit
ATATATAACCGTAGGGCAAATACAGACTGACTTATGTCATCTTTATATAT11.5873015873015872No Hit
GTTACAAGGTGGGACTTTTGTAGTGCAATGGAAGACTGAAAAATGTGGCG11.5873015873015872No Hit
GGTAGCACTTTCTACTCAGACAAGAATCTGTGGTCCACCCAGGCAGCCGG11.5873015873015872No Hit
AGATTAGTGTGGGGCAGGGCAGTGGCACCATGATGTTAGATTATCAATGG11.5873015873015872No Hit
AGCGTGGCCATAGCTAAAACTTTTCCCCTGGCTGGCGTTCCGACCTCGAG11.5873015873015872No Hit
CATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATG11.5873015873015872No Hit
GATTGTATCTGTTCTTGTAGCTTACTATTCCGACAAATACAGCTCTCAGA11.5873015873015872No Hit
CATAAATACAGCTTTGCTCAATGCATCTTGTGCAGCCATGGATGACTTCC11.5873015873015872No Hit
GAATTGTAAGAAGGAACATTTAGTTAGCAGTTCTAATCGGTCTCCTCTTT11.5873015873015872No Hit
GAATGTGACTTACACTGGAACAAGCAGAGCATGTTCAGGTTCATTCTACA11.5873015873015872No Hit
CAATAGGAGCTCAGTGTCCTGCGTAATACGGAATACTTTCATACGTGTGA11.5873015873015872No Hit
ATACTGTTCATTTAGCACAGTTTCTGGCCTATAGAAGGCACACATAAAAG11.5873015873015872No Hit
AGAACAATATCAGATCAACAAAGAGCCGGTTCTTTGAAAAGAACAAAGCT11.5873015873015872No Hit
GGTTGGCACAGGGGCGCGCTCGGGATGTCCCAGGGCCGCCCACACGGTCT11.5873015873015872No Hit
GGATGACTTCCAACTGATTCCAATGATAAGCAAATGCAGAACAAAAGAAG11.5873015873015872No Hit
GACCATGGGCTGGCATTACCACAGCATTGTTGACGGATTCAACTTCCTTA11.5873015873015872No Hit
GCTGCCCAGTGCTGCTGCTGTTCCCATGGCTGAACCGGACACGGGGGCAC11.5873015873015872No Hit
ACGGAATACGTTAGTTAAAAAAACCCAAAGAGGGAAGCCTGTTTTGATCA11.5873015873015872No Hit
GAGAAGCAATCAGCACCAATACCATAGGGAACTTTAATACCTCACACTTC11.5873015873015872No Hit
AGCTGTGGGAGCAGACCCGCTCAAAGGCAGGACTGTTGGTTTCAGATGGA11.5873015873015872No Hit
GATTTCACGTCCGCCCTCTACTGTGACAGACGCAACCTCCGGAAGGTCCC11.5873015873015872No Hit
TTGTACATACTGTTAAATTGATAGAATGAAGAGCTGGTTATATGGACAAA11.5873015873015872No Hit
AGCCACTTCCAGGAATGCGTTTGGGGATGGGGCTAGGGAATAGGGAAAAA11.5873015873015872No Hit
CAATCCGACTTTACTTTTCTTAAAATTGACAGCGCAAAATGAAATAAGTA11.5873015873015872No Hit
CGGCACCTGGAGCAGTTGAGGCTCAGCCAGAACCAGATCTCCAGAATCCC11.5873015873015872No Hit
GCTTGCATACACCCTCTCTCCCTCTCTCTAAAATAAATAAATATTTTTTT11.5873015873015872No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA11.5873015873015872No Hit
GACCCAGCCAGCACCAGCCGATGGTGGCAGGTGTCTCAGGTCGAGTTCAC11.5873015873015872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA50.0145.02
CTTTACT50.0145.09
CAATCCG50.0145.01
ACTTTAC50.0145.08
TCTCCGA50.0145.0145
ATCCGAC50.0145.03
TCCGACT50.0145.04
GACTTTA50.0145.07
CCGACTT50.0145.05
CGACTTT50.0145.06
Produced by FastQC (version 0.11.5)