FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4884731
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC60020.12287268224186755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG7550.046.094449
CGAAAGC26100.036.393983
AGCGAAA28350.033.249121
GCGAAAG31000.030.8746512
CTAGACG1952.6076304E-729.7477473
TAGGGTA19400.025.4164455
TACGGAG2301.1037337E-625.2196257
GGGTACT20150.024.1088357
TAATACA62500.023.899834
GCTAGGG27000.022.2901523
TATAATA58500.022.1864072
ATAATAC54150.022.0945013
ATACAGC51000.022.038076
ATACCGC21850.021.5711826
GTCCTAT44600.021.2973821
GATGCGC8750.020.714858
TGTACCG8800.020.5998925
TACCGCC32600.020.4619357
CTAGGGT25200.020.4298634
TACAGCC51500.020.414437
Produced by FastQC (version 0.11.5)