FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCAAACAAAGAGTACTCATCATCACAAATGAGAGAGATGTTCACTGA12.0408163265306123No Hit
CCTGAAAGGATTATTATAATTAATAGCACATACAAAGCCTGCATCATTTA12.0408163265306123No Hit
ATCTAAGGCCACAATACAAGAAAATAAATTCAAGAAGCCTATAATTTTCC12.0408163265306123No Hit
CACTCAAACAAAATATCACACAAACACACAAAACATCTGCAGCAACCAAC12.0408163265306123No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC12.0408163265306123No Hit
CAAGGAGGCAGAGAGGCAGGCAGAGAGAGAGAGAGAGGAGGAAGCAGGCT12.0408163265306123No Hit
CCTCTACATCTGGTGTCTTGGCTTCCTGATCTGACTCCTCTTATGAGGTT12.0408163265306123No Hit
AAAGTAAGACATAAAAAAATGTTATAAAAAATCTTATTATCAGGGTACAT12.0408163265306123No Hit
CCCTTGTGAAATAAGCTGAAGACTGCAAAGCACCAGACAGCAAGAGCCAA12.0408163265306123No Hit
GACAGAAAGAGAGTGCACAAGCATATGCTAGAGAAGAGAGGGTAAGAGGG12.0408163265306123No Hit
CATAAATCCAGTTGCTGTGGAAATCAAACCTTCCCACTGATGGAGGATTA12.0408163265306123No Hit
GCTTACCCTCTCTGGGCCCCAGTTTCCCCACTGAAGAAATGAGGGTGCGT12.0408163265306123No Hit
ACATAAGATGCAATACTGCCTTCATGGCATAGAAAATTACAAGAGTTAAA12.0408163265306123No Hit
GAGGAGAATGGAGCATGGCTGAAATATGTGAGGGGATTAAGAGGCACAAA12.0408163265306123No Hit
CATGGAAACAAGTTACTCATCAAATTGAGGCTATACAAAATATTGACAAT12.0408163265306123No Hit
ACTGAATATAAGTTATCTACTCTGGCTTCATTTTACAGCAAAACTAAAGA12.0408163265306123No Hit
CTGCTTAACCAACCAACTGAGCCACCCAGGCGCCCTACAGTTTGTTTTAA12.0408163265306123No Hit
ATTTGATACAAGCCCAATAATAATCAATAATTCGCAGAATAACCTTGAAT12.0408163265306123No Hit
TATCTACATCTTGCTCTTCTGTTGGGTTTTGTTTAAGGATCTCTACCATT12.0408163265306123No Hit
GTACTGACAAATAAGAACAAGAAATTCTATTCTCTAAATTGTTATTTTTG12.0408163265306123No Hit
GATCATTCCATACCACTTCTTAAAACACTCAACTACCTTTATAATGCTAA12.0408163265306123No Hit
GTGTGTGTGTGTGTGTGTGTGTGTAGAGAGTTAGGAAAATCTACAGGGAC12.0408163265306123No Hit
CACTAATCACCCTTACAGATGTACCGCTTACCACACAATGTCTCATGTAT12.0408163265306123No Hit
CACTTCCTGCCCAAGGAAATGGAATAAACCCAACAAATGCTCAGGTTCTG12.0408163265306123No Hit
ATGCTAATGAAAACTGCCAATGACGTTAACAATTAGCTCAGCTTTGCTTA12.0408163265306123No Hit
AAACATCTGTGATTCCTCCTCCCCATCAAGTCAGCGGTCGCCTATTTTGT12.0408163265306123No Hit
CAGGCAGCCCTTAGAGCCAACATAAAAAAAAAAAATGTTGTTCTAAGAAG12.0408163265306123No Hit
CTATATATTAAATCCCATTAGATGAACTACTTAGTCGTTAATCAGACACA12.0408163265306123No Hit
ACTGGTCATTAGTCTGTTCATATTTCTTTTTATTTATGATTCAATCTTAG12.0408163265306123No Hit
GAGTGGAGTCCTGAGAAGGTGAGAGTGACCCCTTTGAGCTGGCCCTGGCA12.0408163265306123No Hit
AATAATGAGTTGGTTAACATTGGGGTCATTCAGGGACGGAGAATGGTATT12.0408163265306123No Hit
CTGGACTCCCCGTTGGAGGGAATGAGAAGAAGGCTAAATTGGCAAATGTT12.0408163265306123No Hit
GAACCACACAGTACAATCTGACAGATAAACTATAACGCATTTAAGTTAAT12.0408163265306123No Hit
AACAAACACACACAAACACACACAGAGTAGTATCATTATCCCCATATTTA12.0408163265306123No Hit
AGTCAGATGAGTGATGAGGAGCCCTGGGACCACAATGAGGTAGATAAGCA12.0408163265306123No Hit
AAGCTAACAGATGATCTTCTTATTTTCAGTTCCAATGGAGTAGAAAAAGA12.0408163265306123No Hit
TCCCCATAAGATCATTATAACTACTACATTCTCCCGAGTATTTGGAGTAA12.0408163265306123No Hit
GTCCTCAAGAATCGTTAAATTTTGGCGTTCACTTCTTTAGACATTTGCGA12.0408163265306123No Hit
GGAGAATGTATTAATCACTATTCCAATTTCATGGTGTAGGTGAGCATGAC12.0408163265306123No Hit
ACTGTACACAGCTCATCTCGGCAAAATGATATACTCAGGCTGCACACATT12.0408163265306123No Hit
CTACTGGGATCGGAAGTATTTCCATAACAAGCCGGTTCGTGCACGAAGTC12.0408163265306123No Hit
CTCCCTCTTCTACTGCCTGCTGCTCATTCAACTTTGCTCAGTCTGTAAGT12.0408163265306123No Hit
AAATCCAGTGTACCGGTGGTGTAGCACGAAATGTTCTACGAAGCCGTTTC12.0408163265306123No Hit
GCCCCGTGCAGATCCCACTGGGTTATGTTGCGCTGCTGCTTCCCCCGCCT12.0408163265306123No Hit
AAAATGAACAATGAAAAATAAAACCACAAAAAGCTGCATAATAAAAAAGA12.0408163265306123No Hit
GCCCTGCTCTGGAAGTCAGGAGACCCCCCTGCCCCGGGTTTTCTCCCGGG12.0408163265306123No Hit
CTTGATAAGCTGTGTACTCTTGGGCAAGTCAATAACCCTCTCTGAAACTC12.0408163265306123No Hit
ATATAAAAAAAATTAATGTAAAAAGTAACCTATAAGCTATATAACAACAA12.0408163265306123No Hit
GTAAGCAAGGTAACAAGCCAAATTAACCAATGGGCATTATATGCTCAATA12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)