FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4646972
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG951.0295914E-438.157127145
GTTACGT4600.037.8298071
ACTACGC6550.033.209371
TATACTG30100.028.1841375
CTACGCA7950.027.3611812
GTTGACA37850.025.28651
GTAGCAT51900.025.0072751
CGAAAGC23000.024.2741243
GTTATGT36150.023.667611
ACCGTAC2802.3442226E-723.3058621
TAGCATG49250.022.3778362
AGCGAAA24400.022.2870271
GCGAAAG24850.022.1752192
ACATGGT42600.021.956625
GTATAAG16400.021.6637151
GCTACTA10400.020.9139449
CTACTGA46450.020.6050476
GCGATTA4251.4733814E-920.470177145
GCATGTT56400.019.412374
TTGACAT49200.019.1583882
Produced by FastQC (version 0.11.5)