FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6169700
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA31800.028.0479581
GCGAAAG33850.026.1351132
CGAAAGC35450.024.9555323
GTCTACG4850.023.9222181
TATACTG26850.023.4958525
TGATCCG6650.020.7146599
GTGATCG9600.019.6357699
ATTATAC30500.017.8313663
ATACCGC19500.017.4776486
GTATTAG25550.016.4611571
TACACCG11500.016.3942575
GTATAAG23750.015.5714771
ACTACGC6158.4892235E-915.3282111
TACCGCC33950.014.9489647
ACCGTGC17150.014.7958238
AATAGCG4451.9599436E-514.6655635
GTATTAT50950.014.6594031
GTATATA32250.014.3904211
ATATACG5556.8270674E-714.3719574
TATATAC38350.014.1814013
Produced by FastQC (version 0.11.5)