FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4646407
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC6850.041.282041
GTGATCG7100.027.5705749
CTACGCA11350.025.5538252
TATACTG23400.024.169925
GTTACGT4251.8189894E-1223.8850561
GTAATAT17400.022.5025441
GTTGACA32900.022.2593571
GCGAAAG24650.021.4732482
TAGTGCA34550.021.4038819
AGCGAAA24300.021.1855241
CGCACTG14750.021.138445
TAATCGT2755.2171727E-621.0910249
GTATTAT48200.020.9100421
TGATCCG3551.01666956E-720.4226479
GTATAAG13950.020.271111
ACGCACT15800.019.7336714
TACGCAC15000.019.335733
GGTAGTG33650.019.176067
TAAGGTA35300.018.8977284
CGAAAGC27750.018.5518473
Produced by FastQC (version 0.11.5)