FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4909710
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC70060.14269681916039847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG17250.042.8738442
AGCGAAA23600.029.4944691
CGAAAGC26150.026.6183363
GTATAGG11950.024.2702031
TATATCG2653.7816262E-621.8889775
GTGATCG8650.021.7923079
TATACTG27600.021.0165925
ACTACGC4752.728484E-1019.8440861
TCTATAC67700.019.5994453
GTTCTAT72050.018.8186721
CTATACG3101.4675508E-518.7113534
CGTACCA1950.006840334318.59165
GTTGACA38550.017.868191
CTATACC72350.017.7382854
TATACGG4104.6214518E-717.6846925
TATACCA75600.017.5513555
ACCGTAC3352.862208E-517.3132218
CTAATAC27500.016.874413
TTCTATA83800.016.6126372
ATACCGA5703.108653E-916.536746
Produced by FastQC (version 0.11.5)