FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1802_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1802_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5720536
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC236780.41391226276698545No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT129230.22590540466837375No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA107660.18819914777216679No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA104030.18185358854484965No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG95690.16727453511349286No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA90890.15888371299472637No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT70120.1225759264516472No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG68880.12040829740429919No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA68640.11998875629836085No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG65650.11476197335354588No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA65440.11439487488584986No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA64270.11234961199440054No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT63910.11172030033549304No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC63570.11112595043541375No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATATCATAGGGAACT63300.11065396669123313No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT60910.10647603651126399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA49100.050.503661
GCAGTGC46000.048.863221
ACATGGT51200.047.7249835
CAGTGCA51000.044.641392
TTGACAT58100.042.430782
AGTGCAG53950.042.334783
GGTCCTA74950.041.401519
GACATGG59100.041.3447764
GCTACTA16750.040.254299
GTGCAGC60800.037.2073944
ATGGTCC87000.035.8344577
TGGTCCT87600.035.3394858
TCGACGG12500.034.798965145
CATGGTC71300.034.4746446
TGACATG75700.033.044673
TCGCACC4050.032.225214
TACCCAC57450.031.8045122
TGCAGCA51900.031.7135095
CAGCAAC51700.031.4129777
AGCGAAA11150.030.5633681
Produced by FastQC (version 0.11.5)