FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1801_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629195
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT38010.1047339699299707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG11950.035.7995952
AGCGAAA13800.033.1020971
CGAAAGC16250.027.2188953
GTGATCG4300.026.9761339
TATACTG26350.024.2156475
TAGTACT10300.022.5271764
TACGGGG3355.5102646E-821.6413027
TAGTGCA34000.021.1098189
GTATTAT48700.020.5467971
GGTAGTG33600.020.4980477
TAAGGTA34500.019.9663414
AGGTAGT36650.019.5864286
TATTATC27250.018.8923442
GCACCGT25100.018.7773236
ATAAGGT42050.018.2782173
TATAGCG2000.00791409918.1273372
GTATAAG17000.017.9140761
AGCACCG27650.017.8323175
AAGGTAG42650.017.341045
ACCGTGT27700.017.2739778
Produced by FastQC (version 0.11.5)