FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1801_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948858
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT59780.20272254547353585No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC47840.1622322946713609No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG34990.1186561034814155No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG32480.1101443338404223No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC30700.10410809879621197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT4250.059.713471
TTACGTG4650.054.576832
GCTACTA6250.038.2796759
GTTGACA23850.035.2665371
TACGTGT7400.034.29493
ACGTGTT7800.032.5361864
GTTATGT25100.032.354711
GGTGCGA1851.6455851E-731.3510828
ACATGGT26850.031.056095
CAGTGCA20900.030.5301932
GCAGTGC20050.030.3779281
CCGACTA7850.029.5578984
GTGCGAT2003.2582102E-728.9997529
TAGCATG33250.028.567542
CAGCTAC8750.028.1726197
TGTTGGC39150.027.9641487
GTGCAGC22850.027.607454
CTACTGA33650.026.9350726
TAGCGCG2259.1196307E-725.77712145
GACGACT3709.094947E-1225.4762124
Produced by FastQC (version 0.11.5)