FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1801_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13333
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG150.11250281257031425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCC100.007012947145.04
CGGGAGT100.007012947145.04
GGAGTGT100.007012947145.06
GGGAGTG300.001893871772.55
GGGGTGG450.00943292348.3333321
TTTTTTT4051.1887727E-45.728395585-89
Produced by FastQC (version 0.11.5)