FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1801_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589405
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT130630.2846338468712175No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC126820.27633211712629413No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC72080.15705739632915378No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT70790.15424657444701437No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC70070.1526777436290761No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA60900.13269694001727894No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG60790.13245725753120502No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA60110.13097558398092998No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC59180.12894917750775972No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT51610.11245466460249205No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC49840.1085979555083938No Hit
GTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCA48800.10633186654914963No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG46260.10079738005253404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG20950.041.8806085
GTAATAT18600.033.9170381
GTTACGT5250.033.148471
AGCGAAA17100.032.2276841
TTAGGTA23800.031.6860434
GCGAAAG18150.031.1619662
CGAAAGC20050.028.5706143
ATTAGGT27850.027.3382643
TTACGTG6450.026.9810222
GACTACC4850.026.9065145
GATTAGG30650.025.5505452
GGATTAG30650.025.314241
GTTATGT24450.024.9121971
TAGGTAG36150.024.0704695
ATACTGT32550.024.0593766
CTATGCG17000.023.0333144
GTTGACA29300.023.0158461
GTAGAAA26700.021.4547122
TACTGTG42900.021.1255267
TATTATC25200.021.0053162
Produced by FastQC (version 0.11.5)