FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1801_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923693
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC154510.3138091672246828No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT115900.23539241784571052No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT81330.16518089165997962No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC80580.16365764478004619No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA70540.14326644654733753No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC66360.13477688393650863No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC63360.12868389641677497No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT60420.12271276864743597No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC57010.11578707283333872No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG56110.11395917657741862No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA54170.11001904464799084No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT52640.10691162101292669No Hit
GTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCA51120.10382450733626163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT3650.051.650671
TATACTG20950.037.379655
TTACGTG5250.037.290272
GTAATAT17700.030.7244051
ATTAGGT24550.030.7165853
AGCGAAA22350.029.5229241
GTTGACA28250.029.5171811
TTAGGTA26100.029.170234
TAATCGT2354.3048203E-827.7659669
ACCGTCG1050.00771132827.6190598
GTGATCG3153.45608E-1127.6190579
GCGAAAG24950.027.6085852
GTCGGCT5800.027.5036436
CGAAAGC26050.025.886083
GATTAGG29800.025.7917442
ACATGGT32000.025.6049525
GTACTAG2559.4898496E-825.5916231
CTAGTAC3151.035005E-925.3205553
TACGTGT8150.024.911023
GGATTAG32800.024.538311
Produced by FastQC (version 0.11.5)