FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1801_d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTCAAGAGTAAAGATGACCCAGTAGAGATACAACATGTTGATTAGAT22.8169014084507045No Hit
GGCCTTGCCGGTTCGGTGCCCTCTAAGCCCAGCCGGGGTGGGCCCGTTGG11.4084507042253522No Hit
TGCTTAACCAACAGAGTAACCTGGGACTCCTGCATTTTATTGAAATTATA11.4084507042253522No Hit
ATGGAGGACGTTAACATAACAAATCATAATTCTATACAGTGGAGTCAAGC11.4084507042253522No Hit
GCATGAGGTCCCTTGTCAGCACGGGGAGCCCCTTTCTCTCTCTTCTACTG11.4084507042253522No Hit
GGTGCCTGCTGGCAAGAAAGTGGGTCCCGCTGGAACTGCCGTACCCACTC11.4084507042253522No Hit
TCACAAGAGAGTAATTATACAAAAAAATATAACAAGTCTATTTATAAGAA11.4084507042253522No Hit
ATTTCCAGTTTACTTCTTGTAATTGATTTCTAGTTCTATACAACTGTGGT11.4084507042253522No Hit
TCTCTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGGGG11.4084507042253522No Hit
TGTGGAGAGCTCGCTCAGATTGATACGCTCAGATTGGTCCCACTGAAAGG11.4084507042253522No Hit
GTAGAGTGTTTCCAGGAGCAGTGAATGGTATGTACCAGCATTCTTTTCCC11.4084507042253522No Hit
GGTTATTGAGTTAGGAAATTCTTCAGGGTCCCAGAATGGTCACAAATGGA11.4084507042253522No Hit
GGATGTGCCTGTTGTATGGAGCCTGCCTTGGGTCATAAAATGTGAAAAAG11.4084507042253522No Hit
GAGCCAGGGGTGAGGGTACGCCGAGGGCGTTGAGGGGTTGGGTGGCACTG11.4084507042253522No Hit
GGCGTTACCCCCCTGCGCCACCCAGGCACCTGTATAAACAATTTTAATTA11.4084507042253522No Hit
ACCATATCCTGACTCCCAATACGAGAATATGTATAAAAGTGCTTTATAAA11.4084507042253522No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA11.4084507042253522No Hit
GGTGGGCGAAGTAAAAAAACGAATAGAGGCGCAGTTGACAGAGAGAACAG11.4084507042253522No Hit
GTCTGGGGGAGCGGAGAGCTCTCTAACTGGGCCCCCAGGCAGTGTGCGTC11.4084507042253522No Hit
GTGTACTCATAAGAAGAAATTTGAAAACAACCCCACACGCATAAAGCGAA11.4084507042253522No Hit
ATTTCAGAGTAAATCTCAACATGTGAAGTTGACCCGCAAACAGGGCTTAT11.4084507042253522No Hit
ACTAAACACCACCAAGGTGGTGATCAATCTGTCAGCCTGTTGTCTTCAGG11.4084507042253522No Hit
TAAACAAGGTTACTCATTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTG11.4084507042253522No Hit
ATTGCTCATAGTACATTCTTAAGAGACTTTGCAGATCAATGGTTTCAGGG11.4084507042253522No Hit
GCTGGGCTTGCCAGAAGTTGCTCATCAATGTTTACATCATTTAAGTCATG11.4084507042253522No Hit
ATCCCCGGACGCTGGGATCAGGCCCTGAGCCTCAGGCAGCTGCTTAACCC11.4084507042253522No Hit
GGTTTCTTATTTGTAAAACGACTATGGCCACACTACTTTGTCTAAGTCCG11.4084507042253522No Hit
CATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGTC11.4084507042253522No Hit
GCTCAAGGATCGGGTCCTGAGACCACCAACATCAGGAGGTTCCATCCAAA11.4084507042253522No Hit
CACCATCACAATCAAAATAACAATGGAATTTGTCAAAGTGTAGCAACAAA11.4084507042253522No Hit
GTCAAATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTG11.4084507042253522No Hit
TTTTTCTTCTGTGCAACCTCCTCTTCTGGTTTAGGGACAATTTGCTCTTT11.4084507042253522No Hit
CATGACAAGGCAGCATCATTCAGGATCATTTGCTGGTGGGTTTTTTTTTT11.4084507042253522No Hit
ACCCACTTATGCTAACACCAGGAGTTGTAAATACTGAGAGGGAGGTCCCC11.4084507042253522No Hit
GGTGTGACCTCCCCGCCGGAGGCTACAAATGGACGAGGCTCAAGGATCGG11.4084507042253522No Hit
CCATGACTCCCTGTCAAAGCCCTTTGTCTCCTTCTTAACACTTTTGTTAC11.4084507042253522No Hit
GGCAGGCAGAGGGAGAGGGAGAAGCAGGCTCTCCACAGCGCAGGGACTCC11.4084507042253522No Hit
GCTCCACACAGAACCCTCCATCCAACAGCAGCCAAAGGAATATACTCCTC11.4084507042253522No Hit
GGTGGTGTTGCGCAATCATTTTCATAATTGGGCAGTATCTCCTAGGTTAT11.4084507042253522No Hit
GATATGTTCCTTCTATTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCC11.4084507042253522No Hit
GTGGGAATGCTGCTGGAGGATACCCTCTCTCCCTCTGACCCTCCCTTCTC11.4084507042253522No Hit
CTGGTAAAGCAGTTAGTTCAGATTGAGCTAACCCTCCTATATAACATATA11.4084507042253522No Hit
GCTTCCCTGTGCCACATGCCATGTCACAACACCTCGGTGCGGCAACCACT11.4084507042253522No Hit
GGATGAAGGATCCCATTTATTTTACATCTGCCTGAATAACCTGGCTGTTC11.4084507042253522No Hit
TCACCACCTTGGTGGTGTTTAGTCACTCAATGTTTATTCACTCGAGAGAA11.4084507042253522No Hit
GAAATTTATTTTAGCCCAGTTCTAGAGGCTGCAAGTCCACGCTCGATAAA11.4084507042253522No Hit
GAGGAAAAGGCAGAGGCTTTAACCCACTGAGCCACCCAGGCGCCCCTGGT11.4084507042253522No Hit
GAGACAGACTGAGCAAAGTGGAAGGAGCAGCAGGCAGTAGAAGAGAGAGA11.4084507042253522No Hit
GGTGTATACAGGTGGTGGGGGTGTATGTATGTACACAGTGTCCCTGTGAT11.4084507042253522No Hit
GGCATAAAGAATCTCCAGGAGCTCAAACAAGAGATGGATAGAACTGATGT11.4084507042253522No Hit
GTCCTCTGTCTTGCAAATGCAGATTAGAGCAGCATTCCTGCCTTTCTATC11.4084507042253522No Hit
TATCTATTCAATCAATCTTACTATGCAAATGGTATAGTTCAACTCTAGTT11.4084507042253522No Hit
GCTTTATGGGTATTAGGTGTAAGGTATTCTCTCTAATTCTTAAGCTATGT11.4084507042253522No Hit
CCCTAATCCAATGTGATTGGTGTTGTCATAAGAAGAAATTTGAACACAAA11.4084507042253522No Hit
GAGCATGGGGAAGGTGAGCTCAGGGATGTATTTATTGGTTTTGATCCAGT11.4084507042253522No Hit
GGAAAGAGACGTCCTCCTGGGACGCAGTGCCCACGGTGCTCCGGGAGTCC11.4084507042253522No Hit
CTTGCTACCTCAAGGAAACAGAAAAGACCAAATAAAGTCCAAAGTTAGTA11.4084507042253522No Hit
TTGCTAAAGCAGTAGAAATATTAGTAAACATTGGTTAGACAAAGCTTTGG11.4084507042253522No Hit
CATCGCACCGTGGTCAACCTTGAGAATGTCTGGTAACAACTCATGGTCTA11.4084507042253522No Hit
TCAATGGTTTCAGGGAACATCTCTCTCATTTCTGATGATGAGTCCTCTTT11.4084507042253522No Hit
GGCTTAATCAGAAGTTAGCGATTCCACAATATCAGTAATAAAGCTTTTGC11.4084507042253522No Hit
GGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTG11.4084507042253522No Hit
TGTATCCTCTATGCCCTGTTTTAACTCTTTACGCCCTCTTGAAGTGGCTT11.4084507042253522No Hit
CTTGCCACCGGCCCGCGGTTCCTGCCAGGGCCGGCTCCCCTGCTTTGCGC11.4084507042253522No Hit
GTGTGTGGGTGTGTGTGTGTATTGAGATAGGAAAGTCTTCAGGGTCCCCG11.4084507042253522No Hit
CATGGAGAAGAACCAGCTCGAAGAGGTGCACTCGGCCCTGCCCCGGCACC11.4084507042253522No Hit
TCTCCTTCGAGTGCACACCCTGCATCAGTCAGGGCTTGAGCCACCTGGGA11.4084507042253522No Hit
GTGCCTGAATCTCGTGTGCATCAACTCAGCTCTATCAACATTCTATTCAA11.4084507042253522No Hit
CTCGAGATCACGCTAAAGTTCATACCCATGGATTTACGTTAATTCGACTA11.4084507042253522No Hit
CTATTAGTAGCGGTATTATTATCACAATTATCTTAATCGTCGAGACGTGG11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCG50.0145.01
CCGGACG50.0145.05
ACGCTGG50.0145.09
CCCGGAC50.0145.04
CGGACGC50.0145.06
CCCCGGA50.0145.03
GACGCTG50.0145.08
TCTCCCC50.0145.0145
TCCCCGG50.0145.02
GGACGCT50.0145.07
Produced by FastQC (version 0.11.5)