FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1801_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686172
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA241300.5149192133792785No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA157450.33598852112129046No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC150200.32051747140309833No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG145550.3105946602045337No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA114240.24378106480086517No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA109550.233772896086614No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT103490.2208412324600975No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA94490.20163579143061758No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA89410.19079538693842224No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA86490.18456428829330207No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG86330.18422285823055579No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT83070.1772662207020997No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT77620.16563625918980354No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT75960.16209392228881056No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA69670.14867145294709627No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA67000.14297383877501724No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG63380.13524898360538196No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA58360.12453661538671651No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA55520.1184762317729695No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAAG54390.11606488195482367No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT54390.11606488195482367No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT54280.11583014878668559No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA53940.11510460990334968No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC52470.11196772120186796No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC50360.107465112249401No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG49980.10665421585037851No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA48780.10409349037978119No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG48640.10379473907487817No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG47450.10125535298320248No Hit
GCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC47270.10087124416261291No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA47140.10059383223663151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCA73850.078.058672
GCAGTGC78700.072.971011
AGTGCAG80650.071.9267043
GCTACTA27050.071.828699
GTGCAGC84100.069.061574
ACATGGT57350.065.49325
CAGCAAC60050.065.076587
GTTGACA59150.064.23571
TGCAGCA67050.058.505735
GACATGG66150.057.328664
GTTACGT6050.053.9333081
TTACGTG6100.052.3030932
CAGCTAC40600.047.5011947
GGTCCTA96550.046.2550139
TGACATG86100.045.22473
TACGTGT7100.044.9364623
CATGGTC85400.044.745846
AGCTACT43650.044.512398
GGTTATA12350.042.860361
TGGTCCT105850.042.53358
Produced by FastQC (version 0.11.5)