FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1801_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1801_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33342
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC430.1289664687181333No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG100.00706033145.01
TAGTATT100.00706033145.04
AAGTGCA350.003536308962.1428576
GTGGGCA400.005999848754.3759
TTAATGT450.00955813448.3333324
GAGTATA450.00955813448.333332145
GGTGGTG450.00955813448.333332145
TCATACC450.00955813448.3333329
TAATGTG450.00955813448.3333325
TTCTATA657.234501E-444.6153832
ACCACTG1000.00599164229.08
GGGGGGG10600.08.89151145
Produced by FastQC (version 0.11.5)