FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1800_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1800_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252674
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC6050.035.95561
GTGATCG8400.031.935799
TATACTG36200.027.8429455
CTACGCA8150.027.5806662
GTTGACA37200.023.9752331
GCGTAGT2751.9692015E-723.7306963
GTTACGT5850.022.3109111
GCGAAAG20200.021.8967252
AGCGAAA20400.021.3266091
CGAAAGC20950.021.1128333
CGCACTG12750.021.0426545
ACATGGT45900.019.1153225
ATACTGT51350.019.0634826
TTACGTG7300.018.8725872
GGTAGTG41450.018.716247
AACGCGC1950.006842418.5906567
GTATTAT77800.018.5470221
TAAGACC39000.018.4066774
TGATCCG3552.2923014E-618.381019
TATACGG2400.001059365118.127965
Produced by FastQC (version 0.11.5)