FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1800_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1800_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGGTACCTGGGTGGCACAGTCAGTTAATCAGCTGACTCTTGATTTT13.571428571428571No Hit
CCTTTATTCCCTATCTGATTCTAATTTACTGTGGTAAAACTGTTTCCTCT13.571428571428571No Hit
CCTAAATGCTGCCAAATGCAATCAATTTCAGAAAAACATTATCCAAAGAC13.571428571428571No Hit
CTTTTCCAACCACAGAGGTATAGAACTAGAAATCAATTACAAGAACAAAA13.571428571428571No Hit
GATTATTTCTATGAGGATGGACCAAGATCAAGAAAAATTGCTTAGTTTTT13.571428571428571No Hit
GTGATTGCAGGCTCAGTTCCAAACTTACATGGTGCAAATAAAATACCCAT13.571428571428571No Hit
CTGATGGAGACACTGCGGTGCGGAAGACACCGGCCGCCCGTCGACAACCA13.571428571428571No Hit
GATCACAACAGAAACATCAACCTCAGACAACCTGTTACTTAGATGGACAT13.571428571428571No Hit
AACTTGGTTGCTTTCATCATTAAACCAATATTACCATCGGATTAGCTGTA13.571428571428571No Hit
AAGATGGAAACATACTGCATGTAACGTTGTAGATAGGCTTCTTATACTTA13.571428571428571No Hit
TCATTACACTGTATTTTCAGAGCCAGGAAGATCAATGCGTTCATGCATAA13.571428571428571No Hit
ACAGAACAGATAAACAGCCGAGATAAGTGGAGACTCAGCTATGGTTTTCT13.571428571428571No Hit
GATTAAGGCACTGCAGCAAGTTCTTAAACCTTTGTCTAAAGTGGCTATCA13.571428571428571No Hit
CACCTGGACATCACATAAACTACACTGGATTAAATAAGCAAAGATAACAG13.571428571428571No Hit
TCGCTGTATCGCTTCCCCCCTCCGTTTCCAGATGGACACAAAGGCCCACA13.571428571428571No Hit
GCCATAACCATGAGTGATAACACTGCGGCAAACTTACTTCTGACAACGAT13.571428571428571No Hit
CCCTACGACTTAGTATCGAGAACTCATCAAATGTTAACAATCATCTCCCA13.571428571428571No Hit
GGCAAGGAGGAAAACAAAACAGAAAAAAAAAAAAAAAACAACTAATTGTG13.571428571428571No Hit
CGTCTGCACAGTCCCAGGACGGACACGAAAGAGTGACAAGGACAGGAGAG13.571428571428571No Hit
ACATGTAACCATCCAACAGGACACCAACAAAACCCACACATGTGCACAGG13.571428571428571No Hit
ATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGCG13.571428571428571No Hit
AAATAAGAGTTTCTAGTTATCAGATCCAGCATCAAGCCTGTGCTTGCCTC13.571428571428571No Hit
CCACCATTGCATCCCAGACATACATCTTACTGGATCATACATCGTCCCGT13.571428571428571No Hit
CTCCTCATCCCTGGCAAGAATCCTGTACCGGAATTTATAAAAAATAAGAT13.571428571428571No Hit
TACCAGGGCTGTACCCTATTACAACAATAAATGTATAAAATGACAAAAGA13.571428571428571No Hit
GTTCAATAAAACACTAAAAATAGACTAATCATATAATCCAGAATCTCCAG13.571428571428571No Hit
AGGAATGAGAATACTTGTAAGGGGCAATTCTCCTGTATTCAACTATAACA13.571428571428571No Hit
CATTTAGAGAGGTCAAGTTCAGACTTCATGACCTTCTAGGATCCCAGGGG13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)