FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1800_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1800_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439565
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT73260.1346798870865593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT4250.037.5328981
ACTACGC12300.034.7796551
TATACTG27900.034.5644575
CTACGCA13300.032.164642
GTTGACA34250.029.426091
GTATTAT63600.029.18511
GTAATAT25800.025.8551161
ACCGTAC2901.1919838E-825.0023231
TTACGTG6550.024.3534072
ACATGGT41850.024.0825065
TATTATC44250.023.7592682
GTAGCAT54300.023.2342031
CTAGTAC6600.023.0705363
AGCGAAA27000.022.557651
GCTACTA7500.022.2339679
GCGAAAG28400.021.9562642
TACGCAC19600.021.8262063
GCAGTGC30450.021.668681
CCGACTA9550.021.2587154
GTATTAG15450.021.1184671
Produced by FastQC (version 0.11.5)