FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1800_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1800_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510389
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT59950.13291536494967507No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC45190.10019091479692771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG24950.042.140485
GTAATAT21600.033.905091
ACTACGC9200.030.7378861
CTACGCA10100.028.7164692
AGCGAAA23400.027.5785471
GCGAAAG24950.026.7368162
GTATTAT52550.025.526791
GTTGACA25950.025.427361
ATAGCCG2051.2914277E-524.7564548
GTTACGT3851.6370905E-1124.483861
CGAAAGC27900.023.9098073
TATTATC35650.023.3900262
GTAGCAT44150.023.3214131
GTATACG3753.074092E-1023.2031631
ACCGTAC2852.779634E-722.897861
TAGTGCA47200.022.7336319
TAAGGTA46200.022.6004874
TAGCATG37200.022.4154432
ATACTGT42600.022.1279226
GGTAGTG49200.021.367897
Produced by FastQC (version 0.11.5)