FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1800_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1800_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150587
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG52620.1670164956562063No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA47020.14924203013597148No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC42900.13616510193179876No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA41290.13105494309473123No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG39700.12600826449166458No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT39700.12600826449166458No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA38770.12305643361062558No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA36640.11629578868953627No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT36050.11442312178651154No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA34480.1094399234174457No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG34440.10931296294944402No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA33180.1053137082073912No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT31950.10140967381633963No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT31730.1007113912423304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC4650.046.7804451
GTTGACA26500.045.147541
GCTACTA10500.044.1907739
TTACGTG3300.043.944572
GTTACGT3250.042.390281
ACATGGT28400.042.127115
GCAGTGC27800.037.81981
AGCGAAA11250.036.7382431
CTACGCA6250.035.9642372
CAGTGCA29650.035.948582
TAGTCGG5650.035.9294478
TTGACAT33900.035.2917672
GCGAAAG11750.035.1743552
AAGGTCG5800.035.0002339
TATACTG27400.034.402415
CAGTATG11150.033.8118829
GTAATAT12500.033.6444931
GACATGG35600.033.6064834
GTAGAAA19800.032.9584272
GTTATGT18000.032.6293641
Produced by FastQC (version 0.11.5)