FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1800_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1800_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534471
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC119450.26342653861938914No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA105120.23182417530071314No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA88740.1957008877110472No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA72010.158805735001944No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG68640.1513737765662191No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT63760.14061177147235035No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT63730.14054561160497003No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA62980.1388916149204615No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG53660.1183379494543024No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA50840.11211892192055038No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT46950.10354019245023288No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA46480.10250368786127423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG4200.055.2508162
GTTACGT4450.053.7758331
GTTGACA42800.053.2009121
GCTACTA10400.052.2841579
ACATGGT45700.048.8739365
CAGTGCA38700.047.96972
GCAGTGC39650.047.0027351
AGTGCAG40100.046.2949453
ACTACGC5850.044.6251641
TATACTG24250.040.9686475
GACATGG54750.040.794784
GTGCAGC46000.040.3571174
TGCAGCA34100.038.7043535
TTGACAT59750.037.5023572
TGACATG63000.036.6036643
TACGTGT6550.036.535133
TACCCAC51600.035.9772762
GGTCCTA74750.035.595669
GTAATAT19050.035.0207981
CAGCAAC38750.033.8678327
Produced by FastQC (version 0.11.5)