FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1797_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1797_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715067
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA46340.12473530087075146No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG41970.1129723905383133No Hit
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT39180.10546243176771779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACT3650.039.728434
GTAGCAT37300.038.4875871
ACCGTAC2109.094947E-1237.9784971
TATACTG21850.037.828385
GTTGACA24200.037.1510121
GAGCTAT13550.036.9190678
TTACGTG3950.036.7110862
GTTACGT3900.035.3226551
TAGCATG33500.034.8453982
AGACGAC4950.032.224183
GTTATGT25900.029.6736151
ACATGGT29750.029.4891835
AGCTATC17400.029.1676489
CCGACTA8550.028.8321594
GCTACTA5750.027.7400679
CTACTGA39750.027.5429216
GCATGTT43800.026.6511574
ACACCTA36700.026.4729941
GTCGTAA1100.0096720426.3652361
GCGATTA1958.764218E-626.025116145
Produced by FastQC (version 0.11.5)