FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1797_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1797_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTTATTCACTCGAGAGAAAACACTTGCACATCACAGCTTAGCGTTCT10.7462686567164178No Hit
GATAAAGATAGAGAAAAGAGGCAAGAAAAGATAAGAAACTATCCAGAAGG10.7462686567164178No Hit
GGCACTACAACTGATAATTGAATTAACGCAGTAAAATTATCCAACATTTA10.7462686567164178No Hit
GTACAGTCTTTCCTCAAAAAAACAAAAATACAAGTAACAAATGATAAACT10.7462686567164178No Hit
GAGATAATAGATTACAAATTTGACATAATCTCAAATAAACTCATGGGATT10.7462686567164178No Hit
GGTGAGGGTGAGCCATCCTGGCAGAAGCCACACATCAGCTGTGTCTCAGG10.7462686567164178No Hit
TCACAACGGAAACATCACCCTCAGACAACCCCATACTTGGATGGACTTGA10.7462686567164178No Hit
CACCAAATGTTCCATACATTAGTGAAGCTTTCTCTAACAAGACTAACTAA10.7462686567164178No Hit
GTGCCCCCAGTTCTGTGGAGTCCCCCGAGGGAGGGAGAACGAGTGGGCGG10.7462686567164178No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTAAGTCCTCATT10.7462686567164178No Hit
GAGCTTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAGGACCC10.7462686567164178No Hit
CTGCAGAACAACTTCATAACTGAGCTCCCCGTTGCGTCATTCCAGAATGC10.7462686567164178No Hit
GTGATGCTCCTGGAGGTGCCCTGGCCACTCTGTGCCCCTTTCCTAGATCT10.7462686567164178No Hit
CCTATGGAGTAAGACAAATGGCAAGGAACCAAAGGCTCCATTCACATAAA10.7462686567164178No Hit
TCCTGTTGCTGTCCAGGTAGAGCTGGTGAATGGACGTGGGCACCTTGGGG10.7462686567164178No Hit
GGGCCTCTGAGGCCAAGATGAGGAGAAGTGGGAGGAGCCAGTAGAGGGAT10.7462686567164178No Hit
GGCTTCCCAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAGGAGGGAAT10.7462686567164178No Hit
GAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGAGCCCTGGCAAAG10.7462686567164178No Hit
GTATGCACTCTGTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGC10.7462686567164178No Hit
AGCACAATATCAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCT10.7462686567164178No Hit
TATTTGGGGCGCGGTATGCGGAGATTTATTAAAATTTAAAATTTATAACG10.7462686567164178No Hit
ACAAGAAACATCTCAAATAAGCAACCTAACTTGATACCTCAAGGAAACAG10.7462686567164178No Hit
TCCCCCAAAAGCACCATTAGCATACAATTTTCAAATTACTTTCCGTAAGG10.7462686567164178No Hit
CAGCAAGATGTTGTGTCTATGAGAACGAAGTTGAATGCTTTGGAAAGATT10.7462686567164178No Hit
ATCCTTCTGTTGGCTATTGTTTTATAACTAATAAAAGTGAGACTGTGGAG10.7462686567164178No Hit
AGGCCCAGGTGACTCAGTTGGTTAAGCATCTGCCTTCGCCTCAGGTCACA10.7462686567164178No Hit
GGCATCAGCTGGACCCCGGGAGGGAGCAGAACCCTCACTGAAGGGTGAGC10.7462686567164178No Hit
CTTCTCTATCTCCCAAACCACACATACAGAGAGTCACCAAGTCTAATGTC10.7462686567164178No Hit
GTCAAATCCAGATCGTTTCACAGGAACTCAACCTAATCGTGTGATTTTGT10.7462686567164178No Hit
GACTTACAGGATATGAGGTCAACATGCCAAAATTAATTGCATTTCTGTAA10.7462686567164178No Hit
AATGATACGAAAACTCCGTTTCAACCCTAGCCTCAGCGATTGAGGTCAGA10.7462686567164178No Hit
GGAATAGTATGCAAATGTTAAAAACTATATTATAAAGTGTTAAAAAGTAT10.7462686567164178No Hit
GTAAGTGGGGAGACCAGGCACTTGAGAGGCTTAGGGTCTTCTCCAGCAAG10.7462686567164178No Hit
CAAAAAATCTCCATTAGGAGGAGATACTTAATCTCTGAGTTCTGAAGGAT10.7462686567164178No Hit
GAAAATGAGAAGAAAAGATTTAAAGAGGTCCCATCCTTCCCAACAATTAG10.7462686567164178No Hit
ACATAGCACCGTGTTCAAACTTGAGAATTTCTGGTAACAACTCATTGGCT10.7462686567164178No Hit
TCTGTGTTCCTATTTTGGTTTGCCCATGAACACAATTGGTTGTTCAGTAG10.7462686567164178No Hit
GTTCTTGATTTCAACTCTCTCATGGTCAGTTATGGTCCATTTTGGTCTGA10.7462686567164178No Hit
TTCTTGGGGAGCCCACGGTCTGACAGCCTGTTGTAGTTAAGGCGAATGAA10.7462686567164178No Hit
GGGCATTGTGCAAAGGGGCAGGTGATACCTGCTTGCTCAGTGGTTTGTTA10.7462686567164178No Hit
GATCCATAGAATCCAGTTCCATTTGTGCCCACAGGTCATTCAGTAACGAT10.7462686567164178No Hit
CCGAAAGCAGGAAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTC10.7462686567164178No Hit
ATAATAACAGAGAAGTGAGAAGGAGGGCAGGTGGGAGAAAGCGGAGAGGG10.7462686567164178No Hit
CGTATAGAGAACTTACTTGCATTCAGGTCCTATTATATTCTCATGTGACA10.7462686567164178No Hit
AGTCTGGGTTAGAAAACTCAAAATTACAAAAAAGAAGTCAGCTGTACAAA10.7462686567164178No Hit
CAATCTGTCAGCCTGTTGTCTTCAGGGACCTGCCATTTAGGAAAACAGGT10.7462686567164178No Hit
ATTGTGGACATTGCTGCTATAAACATTCAGGTGCTCATGCCGCTTTGGAT10.7462686567164178No Hit
GGACAGGCCTTCCTTCTGCCCAGACTCCTTCCTCAGAGTGGAGAACGATC10.7462686567164178No Hit
ACTCTGAATTATGTTTACAAACTAAATTATGTAGTTAAAGGTCTAAGAAG10.7462686567164178No Hit
CCTGGGAGAGAGCCCTGCCCCAGGCTCCACACTCAGAGGGGATTCTGCTG10.7462686567164178No Hit
AGCAGGGGGAGAGGCCGGCCGAGGGAGAGGGAGCAGCCGGCTCTCAACAG10.7462686567164178No Hit
GATTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAG10.7462686567164178No Hit
GCATCCCAGGGAGCGCTGAACAGGCCCAATGAGGCACTGGAAGGGGAAAT10.7462686567164178No Hit
CATAAACACAGAGACTAAACAACATGCTACTGAACAACCAATTGGGTTCA10.7462686567164178No Hit
TATAAAGAGATTGAAGAAAGAAGGCGAAAACAAACCAATAAATCATCCAA10.7462686567164178No Hit
GAGTACCCTGTCCAAAGTCAGCTGTCATCCCCAATTCTGGGATGGCGGGG10.7462686567164178No Hit
GGAGAAAAGGAGAGGTCAGGACCAATCTAGCTATATGTAGCATCAGACAT10.7462686567164178No Hit
GTGCCACCTTAACAAAACAGAACCTTATTCTAGTAGCAGAAATCTTTGAA10.7462686567164178No Hit
ATAGACTTCCGAGGAATCAAAAACATACCCTCCTCCATATTTATTTCCTA10.7462686567164178No Hit
CATTAAGGAGGCCTATTAGTAACTACGCTAGGTAGCCATTATCAATTCTA10.7462686567164178No Hit
CTTCAGGGACCTTCCATTTAGGAAAACAGGTGAAAGTTGGATTTATTCAT10.7462686567164178No Hit
CTCTGAAACTCTGTTTCCATATCTCTAAAGTTATGATAATGATCCTCACG10.7462686567164178No Hit
GTATATATGTTCATTTGTGATGAAGCCAAAAATATGCAATGGGTTAAGGA10.7462686567164178No Hit
TTTAAGTCCTCATTTGGGAAAATTCAAGGCTTCTTGAATTTATAGCATTG10.7462686567164178No Hit
GGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTGAGGCCA10.7462686567164178No Hit
GGTAATATTGGTCTATTAAATATATATAATTTATGGGAGAAGAGGAATAT10.7462686567164178No Hit
ACCCAGACCCCCCCAAATGTTCCCTACTTTAGTGAAGCTTTCTCTAACAA10.7462686567164178No Hit
AGTACACACCTAGCTAAGCGCTCTCATGGTCTCACTTAATCCTCCCCCAA10.7462686567164178No Hit
GAATGTTAAATAAGTGTTTCTATTGAACAGATCCAGCATGATGCCTGTGC10.7462686567164178No Hit
AATTCTAATAAACAGACAGGAGAGCCGAAGAGCATGGGGAAGGTGAGCTC10.7462686567164178No Hit
GTATATTCCTTTTGTTGCTTTTGGATGGAGGGTTCTGTGTGTATCTGTCA10.7462686567164178No Hit
CTGCAGAGGCATAAAGACAAATACAATCAAGACTGAATATGTCTCTTATA10.7462686567164178No Hit
GGCTAAGCCAGTTTCCCTTTGTCTCCTATATCTGAACACCACCCTTGCTC10.7462686567164178No Hit
GCTCAATCTGTCAGCCTGTTGTCTTCAGGGACCTGCCATTTAGGAAAACA10.7462686567164178No Hit
TATGTAGAGCATATATGAACAAACCTAAGAGAAGCAATAAGCACCAATAC10.7462686567164178No Hit
GGTGAGGGAGACAGACAAGCAATTAACTATAATGAGATAATTGCTCTATT10.7462686567164178No Hit
CTCAGCCATGAGCAAATCTCGGCAAGGCTGGCTCGTGGCCCACACCCAAG10.7462686567164178No Hit
TTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGA10.7462686567164178No Hit
CGGTCTGACAGACTGGTGTAGTTCAGGCGCAGGAAGGCAAGGTGGGGGCA10.7462686567164178No Hit
CTCACTAAAATGTACGCCTGAAACTAACATTATGCTGTATGTTAACTAAC10.7462686567164178No Hit
ACAAAAGACTGATCAAGAGATTGAAGCAAGAAGGGGAAAAGAACCCTAGA10.7462686567164178No Hit
GTATTTCATTTATATGAACTCATTAATGCAGGAGGGACTCATTGTTGCCA10.7462686567164178No Hit
CATAGAACCTGGATCAACCTTTACAATGTATGGTAAAAACTCATGCGCAA10.7462686567164178No Hit
TTTCAACAGTTTTCCTAAATGGAAGGTACCTGATGAAAAGAGGCTTACAG10.7462686567164178No Hit
AGATATTTGTGATTACATTAGGGCCCACCCGATGATAATACGATCTCTCT10.7462686567164178No Hit
ATGACAAATAGGTCTCTTACAGAATCGCATTCTTCATGGATTGCTGCGGG10.7462686567164178No Hit
TTTGTACACAGATGGAAGAACCGAGCTTCAACGAGGTCAAGTGTTTTGCT10.7462686567164178No Hit
GGATCACATAGCACCGTGTTAAAACTATAGAATTTCTGGAAACAAAACAT10.7462686567164178No Hit
TCCCAGCAGATCTAAGACGTTGTCAGCCCACTGTGAATCCTGCAGTGGTT10.7462686567164178No Hit
ATTCTCTTGTGCTCAGTCACTAGCTGGAACAGCCAGACCTTGGCATTGCC10.7462686567164178No Hit
AGCCAATAGAGTGAAGGGTCCAGTGCTTACGGGACTGAGCCACCCCTGCG10.7462686567164178No Hit
GAACGGACAGAGAAAGAGACAGAGAGAGAATACAAAAACGTCTATAAAGA10.7462686567164178No Hit
CAGATGAACTTCGAAACTCAGTGTTGTCAGGCCCCTCCCGAGAGCCGAAC10.7462686567164178No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT10.7462686567164178No Hit
TGCCTGTGCTTGCCTCTTTACTATCTTTTCTCTGTGCCGACTTCTTTCCT10.7462686567164178No Hit
GGGTGGAGGGATGGGGTAGCACTGTGAATTGTGTAAGATTGATGATTTAC10.7462686567164178No Hit
ATTTTATCTTACCCTTTGAATATTTGGAGAAAAACCAAAAGACGACATAT10.7462686567164178No Hit
TTTTGATTGACGCAACTGTGAGTATCTCTCAATTACATCTAAATAACATC10.7462686567164178No Hit
TCTCTACACAGCAGATTTAAAAATACAGATTGATAAGGTTGGAACAGGGG10.7462686567164178No Hit
AAACTACTTTCTCCATTCTGATTTTAAAAGAAATTAAAATATTATAGGCT10.7462686567164178No Hit
GATTTTCCATAATTGCTTTTATATATGTCAAATGCTACACGATTCTGGGT10.7462686567164178No Hit
CAATGATTTTCCCTAAGTGCTTTTCTATATCTCAAATGCTACACGATGCT10.7462686567164178No Hit
GGAAGAAAATGAATACAGAAGTTAAAAACATTTCCTACATGACTTCCCAC10.7462686567164178No Hit
CTTGGGAGGTTTCGATAAGTGAAATTATGGACTCCCAAAATGAGGTGTGG10.7462686567164178No Hit
GTGTACTCTTGGGCAAGTCAATTAACCTCTCTGAAACTCTGTTTCCATAT10.7462686567164178No Hit
GGCTTTAACCCACTGCGCCACCCAGACCCCCCCAAATGTTCGCTACTTTA10.7462686567164178No Hit
CCCCAAAGTACAGAGATTACAACAATGAATCCCAGCGGCCCTTTACAGAC10.7462686567164178No Hit
TCTCACCACTTTATGGGCCAAACATCACTATTTATAATTGTATACATTAT10.7462686567164178No Hit
CATATACACGGTAAAGGTCATGTGAGGACACAGCCATCTCCAAGCCAAAA10.7462686567164178No Hit
GGTCTGTTAGGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGG10.7462686567164178No Hit
GCCTGTAAGCTTTTGGAGAACTGGGAGCAAGGCAAAGAAGACAGCGGGTC10.7462686567164178No Hit
TCCAAGGACCCTGGGATAATGACATGAGCCAAAGTAAAAGGCTTTAACAA10.7462686567164178No Hit
TCTTTAAAGAACCTTCTCCTAGTTTCGTTGGTGGGTTGGATTGTTGTTTT10.7462686567164178No Hit
GGTCCTTAGAGATCCCACAATTCAGTTGTTTTCAGACTGTGCCCCCAGTT10.7462686567164178No Hit
CTCCACTCTCTGTCTCCACTAATCTTCTAAAGGTTAACTTCTTTCATTGT10.7462686567164178No Hit
ACTGAGCTCTTAGAGCAGACCAGGATTTCCCATGAATATGAATGCCTCCC10.7462686567164178No Hit
GGTTAGAGATGTTGAAGGAGTTCTTGGGGAGCCACCGTTCTGAAAGCCTG10.7462686567164178No Hit
CAATGATTTTCCCTAAGTGCTTTTCTATATGTCAAATGCTACACGATGCT10.7462686567164178No Hit
TCTTCGAGGAACAGGATGATTTTCTCCCGCTGTAAGTGTGGAGACCAAGC10.7462686567164178No Hit
ATGCTTAACAGACTGAGCCACACAAGCGCCCCTTGTTTCCGGTATTGCAT10.7462686567164178No Hit
GATTCGTAGAATTCACTGGTGAAACCATGGGGTCCTGGACTTTTGCTTGT10.7462686567164178No Hit
AAGTAATAGAGCTTTTGAAAGTGAATGGGAAGAGACGGTGGAAAAGAAAG10.7462686567164178No Hit
CCTCCTGGGATCCTAGAGGCTCTGGCTGATCCGGGCCCACCAGGAGTGCC10.7462686567164178No Hit
GTATATAGATTTATTTTGTTTATTTTAACGATTTTAGTTATTTATTGGAG10.7462686567164178No Hit
TCCCATAGGTGACCTTCCTTTCACAAGAGCAGAATCATATGGGCTACCTA10.7462686567164178No Hit
AGAATAAGGAGAAGCATAACAATAAGAATCACGGGCGCCGCGGTGGCTCA10.7462686567164178No Hit
CCAGTAGAGTTCTGGGGACTCCTGGGAGCAGGGCGGGTCCGGCGACGCAG10.7462686567164178No Hit
GGATGAACGAACTCAATTTTACAAAGTAATTCATCAAAATCCTTGAATAG10.7462686567164178No Hit
GTACTATTATTGTTCCCATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAA10.7462686567164178No Hit
GATCCAGAGGAGGTAAGCCTTGAAACAAAGGTTAGTATGGTTGAGCTCAA10.7462686567164178No Hit
CGTAAGCACTGGACCCGTCACTCTATTGGCTCAGTTCATGACCTCAGGGT10.7462686567164178No Hit
GATACAAGGCGAGAGATAATTGGAAGGCAGTAGTACAGAAAAAGTCAACA10.7462686567164178No Hit
ATTATAATGTGACTGCAGCGTCTTTCATTTTAAATCTATTCTAGATAATT10.7462686567164178No Hit
ATATGTAGCATCAGACATGTGGGTACTTTACAGATGCGAAAAGTAAGTCA10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers