FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1797_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1797_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9938262
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC409520.41206400072769267No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA263340.2649759082624306No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG262330.26395963398831707No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG251260.2528208654591718No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA202480.2037378366559465No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT185930.18708502553062095No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG176020.17711346309847736No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG172460.17353134783526536No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG162460.1634692263093889No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT156480.1574520776369148No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC145080.14598125909741563No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT142380.14326448628542898No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG134690.13552671483203No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA128940.12974099495465102No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT126830.12761788731269108No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA126550.12733614790996656No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC123350.1241162690216861No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC123220.12398546144184969No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC119450.12019204162659428No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA114070.11477862024567274No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT114020.11472830963804335No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT112350.113047935343222No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT110480.11116631861788309No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC110210.11089464133668442No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT109940.11062296405548577No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG109740.11042172162496822No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG108920.10959662765984636No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT108020.10869103672251747No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC107210.10787600487892149No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT105270.10592395330290146No Hit
TCTATGATGTGGGAGATTAACGGGCCAGAATCAGTGCTAGTTAACACATA104200.10484730629963268No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC103880.10452531841080462No Hit
CTTATTAGGTAGCTCTTTTTTGTCAGCTTTTGCTTCTTCTTCCCTATTGT101980.1026135153208881No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC101920.10255314259173284No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC101910.10254308047020697No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA99820.10044009707129878No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG99660.10027910312688476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA19400.0103.533372
AGTAGAA24950.092.127881
GGTACTG22100.091.2149055
GACTTAA3700.070.53963145
TATACTA27850.064.048992
AGGTACT33350.063.2711944
GTACTGA38450.058.840146
CAGGTAC34400.058.5990943
TTTGGAC46650.055.3353773
CGAGACG2000.054.374302145
GACAGTA48100.053.6621327
CTGGACG553.2206098E-452.726593145
GTCCGTT1405.456968E-1251.785046145
AACACTG23500.051.221645
GCGAAAG7650.051.184052
AGAAACA46050.050.5457084
CCCACGC8900.049.690372145
TAACTAT19350.047.591031
CAAGGTA20550.046.5708549
GGACAGT66100.042.454626
Produced by FastQC (version 0.11.5)