FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1797_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1797_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4509259
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC103090.2286184936372029No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA67770.15029076839454109No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG67640.15000247269008055No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA52180.11571746045192792No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG46960.10414127908820496No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC45710.10136920500685369No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT45110.10003860944780506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTAT12100.038.952131
GCGAAAG14150.038.9457742
GGTACTG22400.035.9317325
AGCGAAA15000.034.805221
AGGTACT25150.032.291134
GACAGTA25300.031.236067
GTATAGG55200.031.132391
TATAGCG5650.030.8010852
AAGGTCG10200.030.5645879
TATACTA18100.028.8441095
GCAGGTA27400.027.522382
CAGGTAC28500.027.4778083
GTACTGA33850.027.4194876
CGAAAGC20500.026.8820843
GCGGTAT6500.026.7735466
AGCGGTA6600.026.3675945
GGATAGC26850.026.195745
CTACTAG18900.025.3181729
TAGCGGT7250.025.0037524
CTATACT38150.024.708824
Produced by FastQC (version 0.11.5)