FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1796_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1796_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4932640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19350.045.3396031
GCGAAAG20750.043.3288232
CGAAAGC25700.035.5476343
TATACGG4500.033.8364265
TACGGGG5450.027.9366347
ATATACG6500.025.656194
GCCGTAG4850.025.4144341
TATACTG24450.024.317155
TATCGTC3452.9704097E-923.116657
TAGGGTA14200.022.466845
TATATCG3208.4771455E-720.3924885
GTATATA31250.019.953621
TAGTGCA44350.019.2905259
TAGGACT34800.019.1684174
TATATAC28550.019.0470813
CCGTAGA7450.018.4914361
GGGTACT15800.018.354997
TGTATCG3602.6186372E-618.1266575
GTGATCG4455.549373E-817.922089
ACGATTC2851.6350379E-417.806477145
Produced by FastQC (version 0.11.5)