FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1796_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1796_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5925950
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG29800.030.4170682
AGCGAAA31100.029.1456151
CGAAAGC32750.027.0129573
TATACTG35550.026.721165
GTAATAT29400.021.4583111
GTCCTAT45700.020.3103491
GCACCGT41300.019.840646
AGCACCG41850.018.193555
GTATAAG15650.018.0706541
TACACCG18150.017.1796955
CTAATAC24950.016.857033
CGTAGAC4751.16679075E-716.7927843
GTATAGG14250.016.7927841
GACCGGC14250.016.789242145
CACCGTG59250.016.7641897
TCCTATG52750.016.4961432
ACCGTGT48900.016.4568278
ACTGTGC60200.016.1376518
ATACTGT51850.016.0833446
GTATTAT57450.016.030141
Produced by FastQC (version 0.11.5)