FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1796_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1796_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4549837
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG22050.030.9099242
TATACTG31450.026.2825585
AGCGAAA27550.024.4762421
GTATATA24650.022.9435771
CGAAAGC30050.022.9222773
ATGCCGG6050.020.37157145
GTAATAT30050.019.3031851
ACACCGT17850.019.0916356
CGATACG2359.201131E-418.5123714
GTCCTAC23500.018.2040331
TACGAGT2450.001217062217.7567654
ATTATAC20300.017.501643
TAGTACC18250.017.4810894
GCGATTA2600.001813264416.730495145
TAGGACT43550.016.649094
ACTCGCC3504.1673877E-516.571157145
ACACGAC9650.016.528227145
GTCCTAT37850.016.4746421
GTAGGAC36300.016.3789443
ACTGTGC51350.016.3778928
Produced by FastQC (version 0.11.5)