FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1796_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1796_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4461536
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC74610.1672294026093256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19350.035.225592
CGAAAGC22050.031.569963
TCGACGG6100.030.900972145
TATACTG37650.026.5781965
GCGTTAC3709.094947E-1225.4772781
TCTATAC56950.025.3379063
AGCGAAA27000.025.2450071
GTCCTAT39900.024.170751
GTTCTAT61000.022.3480381
CTATACC68850.021.2742334
TACACCG6900.019.9671425
GACTCGC8500.019.617224145
TATACCA78150.019.1139145
GTATATA23700.018.969451
TACCACT67700.018.955397
GTAATAT18450.018.8649771
ATACTGC22850.018.4059436
TCCTATG49650.018.2558542
GTATACG4054.0603118E-717.904261
GAGTATA54800.017.727718145
Produced by FastQC (version 0.11.5)