FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1796_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1796_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338797
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG34010.10186303629720525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG8150.042.701159
CGAAAGC21600.030.8841443
TATACGG1050.007705495727.6230835
CATAGTG15850.025.1614074
TATACCA22000.023.4011485
TATACTG19050.023.2186185
TATACCG2903.2862044E-722.5032885
GTAATAT14300.021.2971381
CACGAGC7200.021.14544145
TCTATAC23900.020.630633
ATACCGC21250.020.1323556
CTATACC23250.019.9599064
TACACCG8000.019.9404145
GTATATA14050.019.61171
ACTGTGC25300.019.4863878
CTAGCCG6400.019.2586089
GTCATAG20150.019.0722662
GTGATCG3051.2759503E-519.0171789
AGTGATC21950.018.4979027
ATATACT23650.018.3959244
Produced by FastQC (version 0.11.5)