FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1796_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1796_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298873
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC53060.12342769837583012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA21450.036.508611
TAACGAT2008.951247E-932.6303254
GCGAAAG24150.032.4272772
TGATCCG7250.031.0000139
CGAAAGC27100.029.4328483
TCTATAC37650.028.503993
GTTCTAT39600.028.3814961
GTAACGA2354.2977263E-827.770493
GTCCTAT29250.027.5166721
TAGACCG6100.027.3405284
CGTTATA4600.026.797231
TATACTG21900.026.4886515
TATACCA46900.021.4909655
TCCTATG35750.021.2969362
GGGTACT14300.021.294217
CTATACC51850.020.557844
TACATTG12900.020.2330367
ATACCAC52600.019.8513816
TACCACT52600.019.4348727
TTCTATA64000.018.4676442
Produced by FastQC (version 0.11.5)