FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1795_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103174
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3200.043.0521472
AGCGAAA3500.039.3619651
CGAAAGC4600.031.5256023
TACTCGG950.004723623430.531445
CAATCCG1358.0795976E-426.8539269
TATACTG4950.024.9031685
TTGCACG1800.00430881320.137705145
ACCGTTC2608.079113E-519.5189678
GCGTGCC2256.868915E-419.3330757
TATAGAC4954.7293724E-1019.0427362
TAGGGTG3501.996248E-618.6459875
TTACTCG1950.00683615718.5920224
CCGTTCA1950.006837945418.5911799
CAGTACG2400.001059255618.12649
AGCACCG4455.5297278E-817.9243565
GCACCGT4054.0534906E-717.904246
GTATTAA10250.017.6850931
GTATTAG7900.017.4388471
ATAATAC11000.017.1384643
GTCCTAC7351.8189894E-1216.7707611
Produced by FastQC (version 0.11.5)