FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1795_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCTGTTAACTGTGGCGGGACACACGCACCCCGGCCTGCTGGGGGCCT11.2195121951219512No Hit
GGCATCCCGCTGATCAAAGAGACTGATGCGGGGCCTCGGATCAAGGCCTG11.2195121951219512No Hit
ACTGCTGGCCCAGGACAGGCAAGAGAGGCGGGTGCGCAGCAATGAGGCTT11.2195121951219512No Hit
CTCTGCAGGAGGCCATCAGCTCCACATGGCTCATCGTCAGCTCTCAGTTT11.2195121951219512No Hit
CACTTAGCGTTTAGTGTAAGGGTGAGGAATTAGACTGAAGCACAAGGGAA11.2195121951219512No Hit
GCCAATGAAGGGCACTACAACTGATAATGGAATTAATGCAGTAAAATTAT11.2195121951219512No Hit
CTATTTTCTAGAGTTTGTGTGAAATTGCTATTATTTTTCCCCCCAAATAT11.2195121951219512No Hit
GGGGTGGGGCGTTGACAATACCAAAGCGGAAAGAGTCTCATCCAGATTCA11.2195121951219512No Hit
GTAAGAGGAACACTCTCCTACTTGTTACTCAACTCAACTACTGTGTCGAA11.2195121951219512No Hit
GGGGAGGGGGAGGTGATTAGGGTATAGGGATGTTAGTCGTAGGGTACTCT11.2195121951219512No Hit
CGCCGGGGGGGAGCAGTTGTTGCGTGGGCTGCGTGCTTGGGGGGTCCGCA11.2195121951219512No Hit
GGGGCAGTAAATATTTATAAATGTTGGATAATTGTTCTGAATTAATTCCA11.2195121951219512No Hit
TCATAGGACCGTGCCTTCGCCAGACAATAGGCGTCGCGTCATCATAAAAT11.2195121951219512No Hit
GTGTTTATCTGTTAATCAGCTGATTGTCATTTGGGTTATTTCCACCTTTT11.2195121951219512No Hit
GACAGCCAGTGTTTGATTTCTCTTTCCATGAAAGTGTCTGAGCATGGACA11.2195121951219512No Hit
CATAGAATCCCCTCACTCCCACCCAGATGACCATGATTTGTTCAACCATT11.2195121951219512No Hit
GAAAAGGAATGGTGGTGAGACCCATATGGGAGTGGGCTGATAAGTAATAT11.2195121951219512No Hit
GGGGGGGGGTATGTCCGAAAAGTGGTTAAGAAGCGACAAGAGGTAGAGGG11.2195121951219512No Hit
GGCTCACACTGTACAGAATCTGACCAAGGTGCTGGTCCTGACTTCCAGGA11.2195121951219512No Hit
CGGTGGCGCCCTGAAGCCAGCGCCCGGCTCGTGGTTGCTGTGCCCCCCGC11.2195121951219512No Hit
GTAAAATAGAAAGTGAGGAGTGTTCATTTTCGGTTCACAAGATTCGTACT11.2195121951219512No Hit
TTTCTACCCTACAGCCACAGAGCCTGACTCCATCCAACCATCTTTTCCTG11.2195121951219512No Hit
GATTCTATTTGGTGTCCTGCCGTGACTTTGTAATGTCTAAAATTTGAAGT11.2195121951219512No Hit
GATTTATCACATATTAATTGATAAGATATAAAGGCAAAGTGTCTGGCGCG11.2195121951219512No Hit
GGCTCGGGCAGTGCCTGCGCCGCAGGAGTCGAGAGGCGACACCGGGCGTG11.2195121951219512No Hit
ATATGAAACTTATTACTGAAACCAGTAATTTAGCCTAGAAATTCCTTTTC11.2195121951219512No Hit
CCGCGGGGGGGCACAGTACATGGGGGCCATGGAGCGACTTCACTAGGTCT11.2195121951219512No Hit
ATGTCTAACATAGCGTGTATGTGCCGGCCATTTCTTCGCTGACTATATGA11.2195121951219512No Hit
GCCAGGGGCTCGGGAGCGGGGTTGGCCCCGGGGTAGGGTCCGCCCCCCGG11.2195121951219512No Hit
GCCCCCTATCATCGGCGCAAAAGCGTTTCACGTTCCTGTTCGGGATGGCA11.2195121951219512No Hit
GGCAATATATGCTCCCACGGTATTGATTAATTTTGGTACAAAGTTCAAAA11.2195121951219512No Hit
GTGCGAAGGAAGGGCGAGTCCTATGCGCTCTGGCATTAAAGCGATCACCC11.2195121951219512No Hit
GAGACAGACAGACAGAATGAGCAAAGTCCAAGGAGCAGCAGGAAGTAGAA11.2195121951219512No Hit
CTCCTAAAATGAGGTGGGGATCATTCCTTCCTCTTGTTTTTCTGGCAGAG11.2195121951219512No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCAACAATACCATACTTTAAT11.2195121951219512No Hit
CCATAATAAATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCC11.2195121951219512No Hit
GTTGGACCAAATGGGTTTCATCATTGTATTGAGATTATTGATATGCGCTT11.2195121951219512No Hit
GTCTTTTCCCATTGGACTATTTCATTCTCCCTACCCAATTTCCTAAAAAA11.2195121951219512No Hit
GGGAGGAGGAGAACGGGGTGAGGCCTCCCTGGGAGGACCGCAGGCTCGTT11.2195121951219512No Hit
CCTGAGAAGGCAGAGATCTGCTAGACCTCACCCTCATGGGCCTCAATTTC11.2195121951219512No Hit
GAAGCAAACCAAAGAGGCACCGCCAGAGAAGGAAAAACTTTGAACAACTA11.2195121951219512No Hit
CTCCAGTCCTCCGTGAGGAGGGCCCTGGGTTTTCTCCTGCTCTCTCTGCT11.2195121951219512No Hit
GCCGTCCTCGGAACGTAGTGGCGTATGTTAGTTGCAGCTCCGGGTTGGGT11.2195121951219512No Hit
GATCTAAGATGTTAAAGAACTTTTGAGTGGTCAAGGACAAGGACAAAGGC11.2195121951219512No Hit
CTCTCATAGGGGCGCCCTAGCCCGTCAGACGGTAATGGTGTTTAGCAGGA11.2195121951219512No Hit
CGACAGGTCCAAGGAAACACAGCTTGCACACCATAAAACAGGAATGCAGC11.2195121951219512No Hit
CTGGGAGAGGGTGATCATTCTCCTAGTTATTTGATACGCTCTCATTTGGA11.2195121951219512No Hit
TGTATATTACGCTACAGCCCAGTTTACGATGGCAACTTGTCCCCTGAAAA11.2195121951219512No Hit
CTGCGAGGTACTGCTCAAGATTTATCTGTTGGGTACTCCTATGTGGTCCC11.2195121951219512No Hit
GTTTAATCTCAAATGGCTCCAGAAAATAATGAAAGGGTAACCTGGAATCC11.2195121951219512No Hit
GAGCCATTCAATCATAGCCAAGTTCAAAACAAAAGAAAAGAGACTTCGTC11.2195121951219512No Hit
GAATATCACAAAGGAAACATCAGCCTCAGACAACCCGTTACTTAGATGGA11.2195121951219512No Hit
CACTTAGAATAGTAACTATCAGAGTAAGCACTCAAAAAATGTTCTAAAAA11.2195121951219512No Hit
CCCTGGCCTCCAGTGGGGGCTGCCCTGCGAGCAAAACCCCGCGCGCTGCC11.2195121951219512No Hit
AGCTAGGACAGGGCTGTGGGAGTTAGGATGAGATCAGGCCGTGCTGGGGG11.2195121951219512No Hit
CTTTATTTGTATCGAACTAATTATTTATTTCAAGACATAATGTGTAGGGT11.2195121951219512No Hit
GAGGCGAGCCTAGTCTGGCGGTGTGACGTCCCCGGCGCTGGCGAGAAATT11.2195121951219512No Hit
TAATAATGCAAGATTTATTATTGCCCCTAAAAAGATGGATACTGGCTAAT11.2195121951219512No Hit
TCCCAAGCACGAGGGGAGAGAGAACTGTCACACTCACCGCTCCTCACTGG11.2195121951219512No Hit
CTTAATAGAATACAAGAACAATAAACGACCAAGACATATGACAGTGATAA11.2195121951219512No Hit
GTACCTATGAGAGTAAGCCCTCAAAAAATGTTGTAAAAAATCTTAGTCTG11.2195121951219512No Hit
GGCTGACAGACAAACTGACCGGGTCCGATGGATTCAGCACTGATAAATAG11.2195121951219512No Hit
GGGGTGGTGGGGGCAACAGGGAGGAGCGGTGAGTGTGAGAGGTCTCTCGC11.2195121951219512No Hit
AAATAATTGTCAGCTGGAGATAAAAGAAGGGAGAGTTATGAGTGGACAGT11.2195121951219512No Hit
TCACAGCTTAGCGTTCTCAGTGATTTTTTGAAAAAAAAAAAGAAAGAAAG11.2195121951219512No Hit
CCCCATTAAAATAGCATTCAAATGGACCCTTGAAAGAGTCGGCCCTCAGC11.2195121951219512No Hit
GATACACACAGAACTCTCCATCCAAAAGCAGCAAAAGGAATATACTCCTC11.2195121951219512No Hit
GACACAGAAATATGACTGTTCATCTGTGTCCTTAGGAGGATTAGAGAGAT11.2195121951219512No Hit
CAAAAAAGGACTTTCACAAATAGAGGCATGAACTCCCCCATTCATCACAT11.2195121951219512No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT11.2195121951219512No Hit
GCGCTAGGCTGTGCCAACGAAGAGTGTGCCACAGTGACCCCCTCATCTCG11.2195121951219512No Hit
CCCTGCATTTATATGGTAATGGCATTCCTAGAAAATACCATTCATCTAAC11.2195121951219512No Hit
AAATCAATGTTTAATGATAAGAAATTGATCTGGATAAGTAAGAACTTACA11.2195121951219512No Hit
TATATAACCGTAGGGCAAATACAGACTGACTTATGTCATCTTTATATATA11.2195121951219512No Hit
GCTGAGACACTGCGCGCCGATCCCCCGCGGCCTCCCGCCTGCTGATGGGC11.2195121951219512No Hit
GGAAGAAACTTGGCACAGCTGATTAAAGGAAGAAACTTGGCAAAGCTGAT11.2195121951219512No Hit
GGCCACTGCAGGAGCCGGAGCACCAGAAGCGTGCGCTAGACCCCCACCCC11.2195121951219512No Hit
GAATGCATGTTGGTGCAGCCTCTTTGGAGAATAGTGTGGAGATTCCTCAA11.2195121951219512No Hit
GGCCCGTGTCAACCCCGTGCTTACGTCCCTCAGAGAGAGACAGAGAGTTC11.2195121951219512No Hit
GTGTCCTTGTGTTTCTATATGCAGTGTCAGCTGTAGCTCTGTCGCCTGCG11.2195121951219512No Hit
TTAAAATGTACTGCCAATTGTGCCTTTCTAATTAATTTGGCCTTCATTGA11.2195121951219512No Hit
GGTGAAGTCAGAAGCAGGGACCCTGCCCCGCTCTTCATTACGAGGAGCCT11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATC50.0145.0145
GAAATAT50.0145.07
AAATATG50.0145.08
ACACAGA50.0145.02
GACACAG50.0145.01
AGAAATA50.0145.06
AATATGA50.0145.09
CAGAAAT50.0145.05
CACAGAA50.0145.03
ACAGAAA100.072.54
Produced by FastQC (version 0.11.5)