FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1795_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955487
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG8500.028.1478259
GCGAAAG16150.024.694822
ACGCCGA3452.9649527E-923.120376
GAGCGAA6500.022.3116841
AGCGAAA17300.022.2149561
ATTATAC17750.022.0605623
GTATAAG13900.020.8670431
GTATAAT16100.020.7179931
GTATATA24150.020.417731
GCACCGT19050.019.4131686
TAGTACT19000.019.0826034
TATACAT30700.018.4237255
GTAATAC16950.018.3958553
CTAGTAC15850.017.8425353
CGAAAGC21600.017.7927593
TATACTG24900.017.4732275
GTATAGG13300.017.4467321
ATGCCGG13050.017.222038145
TATAATA32050.017.1949632
ACTGTGC42350.016.947878
Produced by FastQC (version 0.11.5)