FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1795_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCATGACCTGAGCTGAAGGCAGATGCTTAACTGATTGAGCCACGTGAC11.098901098901099No Hit
ATATATTCCTTCCTTAAAGGTGGAGTTAATTAATACAATTTGTTGTCAGA11.098901098901099No Hit
TCAGGGGGCGGAGGGGGGCTGGGTGGGGGCCTGGGATGGGGTCTGGGCCT11.098901098901099No Hit
GGCCCCAGCTCTGTACCCATCAGATCGCAGCCCTGATCTCTCCTGCTGAT11.098901098901099No Hit
AGGCACCCTTGTCTTTGTTTTTGTACAAAGAAAGGTGGAATTCAGGAAGC11.098901098901099No Hit
GGAGGCTGTCAAGATCACTGCAGATGAACTTGGAAACTCAGTGTTGTCCA11.098901098901099No Hit
GTGCCCTTCATTGGCTCTTGTTACAGTGTTTGGATTTTTTCTTGCTCTAC11.098901098901099No Hit
ATCCCGGGGGCCTGGGATCGAGTCCCGCATAGGGTTCTCTGCTCAGCAGG11.098901098901099No Hit
GAAGTAAACTGTAAATTCATAAACACAGAGACTAAACAACATGCTACCTG11.098901098901099No Hit
GAAAAGAGAGAGGGAAAACACCCTAACAGCCCATTGCACAGAGAAGAGAA11.098901098901099No Hit
GAATAGGAACTTGGTCAACCCTTCTCTCACCAATCCCAGATGTAAGCTCT11.098901098901099No Hit
TAATCGACTAGACTAATCAACTAATCGGGTGCCCCATTTTAGAACCTGTT11.098901098901099No Hit
CACTGAGCCACCCAGGTGCTCCTACATCATTATTTTTAAACAGTGACATA11.098901098901099No Hit
GGATGATTCGTAGAATTCACAGGTGAAACCATGGGGTCCTGAACTTTTGC11.098901098901099No Hit
GCACAAGTGAGGGAGAGAGCAGGGGAAGGGCAGAGGGAGAGGGAAAGGGA11.098901098901099No Hit
GCTGACAGAGTCTCAGCCATATTCAAAGAAAGAGGACTCATCATCAGAAA11.098901098901099No Hit
AACATGAGCCCTGAGATCTGACAGACCAGATGAAAATCCCAGCTCTACAT11.098901098901099No Hit
AGTATATAGTGATACAGGCCTAACTCAAGAAACAAGAAACATCTCAAATA11.098901098901099No Hit
ACTTATAAAAGTTCCTGTTTGCACACCACTGCTGTATCACTTTCTGATAA11.098901098901099No Hit
ACACCTAACAGACCAAGCCACACAGGCACCCCGTATAGCCAATTTAAAAA11.098901098901099No Hit
GGTTAAGTGATATAGAATACTTAACATTTTTTTTTTTTTTGTTTCCAATT11.098901098901099No Hit
GGTCTATGACACCCGATAGTCAACCACTGCCTTAAAGTTAGCAGAGAAAA11.098901098901099No Hit
TAACATACTAACGTATGCTTACTAGAATGAGAAGGAAGAAAATGAATACA11.098901098901099No Hit
TATGTATTTATTGTAGAGACAGAAAGAGAGAGCACAAGAATATGCTAGAG11.098901098901099No Hit
TGGAAATACACTGAATCAGCTTAAGGAAAAAAATGGGGAATTCTTTTTTA11.098901098901099No Hit
GGCTTGAATGGTGGATTTCAAATCATATTTATCAACACGTCACTTCTTTC11.098901098901099No Hit
CCTCTGACCATTTCTCAGCAGTATCCACAGATGACAAAAATGTCTTTGGC11.098901098901099No Hit
GAGTCAGCCCGTTAGGTGGGCGGATTGCACTATGGGAAAGAACCATAGAA11.098901098901099No Hit
TCTTATATCACATTAGCATAGACACAGGTGTTCATAACGAAATAAGGAGG11.098901098901099No Hit
ATACAATGCCCTTCGAACATGTCTTTGTTTTGCAAGAGTCCTCTGAGTAC11.098901098901099No Hit
GCTGGGTCCTCTGGGGCTGGAGAGGCAGGCCGACCTGGGGCTCAGTCCCA11.098901098901099No Hit
GTGTACATACATGCTTGGTCAATCTTTTATGCCCATTATTTTTTTTTTAT11.098901098901099No Hit
ATTGTATAGACACATGACACTTAAACCAATTTTCTATCATTGGATTTTTC11.098901098901099No Hit
CCTCGTCTCTTTCCTGTTCCCTCTCTGGTGTGAATGGAGTGCTAAGAGAA11.098901098901099No Hit
TTAGAGAATTGATTCCATTTACTATAGCACCAAGAAACATAAGATACATG11.098901098901099No Hit
GGATCAAGCCCTGCCTCTGGCTCTCTGCTCAGCAGGGGTCCTGCTCCCAC11.098901098901099No Hit
CAAATATACTCCTCCACCCCTTTCCAACCATGTCTCCCGGCATCCTGGGC11.098901098901099No Hit
GTTTTGAGGAGGTAGGATTACACAGTAGGAACAACTGAGGCAAACTGTGA11.098901098901099No Hit
CAGCAAAGAGCTGTTTCTTTGAAAAGAACAAATCAGACAGAGTCTCAGCC11.098901098901099No Hit
AGCTAATAGAATGGTATTCTTATGGTTAACTCAGAAATTGTAATACACAC11.098901098901099No Hit
GTGTGGACCCTTCCACACATATTTGAATGGGCTGTATAAAGCATGTCCAA11.098901098901099No Hit
ATACCATAGGGAACTTTAATACCTCACACTTCCCTCAATGATCCCCCATC11.098901098901099No Hit
GGGGCTGGGGGAGACGCAGGCTGCGCGCGGAGCTGGGCGCGCAACTCAGG11.098901098901099No Hit
GGTGCGGGTGAGCCATCCTGGCAGAAGGCACACATCAGCTGTGACGCAGG11.098901098901099No Hit
GAGCTGGGGAGAGAGACAGACAGACTGCGCAAAGTGGAAGGAGCAGCAGG11.098901098901099No Hit
CCCCATGCTGACCTGGGACCTCATTCAGGGCTCAAATCAAGGCCCATAAA11.098901098901099No Hit
ACTTAGAGCAGGGGTGTGCTTTGTTCATGGCTGAAGAACTAACAGATGAT11.098901098901099No Hit
GGGGCACGCTCGGGGTGTTTTCACTCCACTCATGGGAGCTATGGACAGTG11.098901098901099No Hit
CCATTGTTGGGGCTCTCGTAGTTAATATCTACAGTATAAATTAAGGTAAA11.098901098901099No Hit
GGATAAGATTTTTAAAGACATATGTATTAATTGTAGAGACAGAGAGATCA11.098901098901099No Hit
GGGTGGCAGTGATTTGGTAGCGGCGCTATCAAATGCTTTTTCGGCATCTG11.098901098901099No Hit
GGGCGGACCGGTGTGTCTTGGCAGATATCCGCATCTGAGTCCCTGGATGC11.098901098901099No Hit
GTTAAGCATCTGACTCTTGATTTAATACATACATACATACCTGTGTGTGC11.098901098901099No Hit
GAGTAAGCCCTCAAAAAATGTTGTAAAAACTCTTATTCTGGGGGTACCTG11.098901098901099No Hit
GGGCAGTGTGGACGGTGACAGTAATAAACAATCCTCCAAGTAGTTACTAC11.098901098901099No Hit
GTATTGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATTT11.098901098901099No Hit
GAACATAGCATCCATTGGTCTAGCCTTCCTGTACCCCCGCTTTAGGACTA11.098901098901099No Hit
CCCCAGGACCCTGATATCATAACCTGAGCCTAAGGCAGACTATTAACAAA11.098901098901099No Hit
GTGATCTTCTGTCTGGATGGGGGATCATTGAGAGAAGTGTGAGGTATTAA11.098901098901099No Hit
GTTATGTGTTGGCCATTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG11.098901098901099No Hit
ACACGGCACTTCTTTCAAATAAATGCTTTGGCCAAAACTCTCTACACAGC11.098901098901099No Hit
TAGAGCAGGGGTGTGCTTTGTTCATGGCTGCAGAACTAACAGATGATCTT11.098901098901099No Hit
TCTTATATCACATTAACATAGACACATGTGTTCATAACGAAATAAGGAGG11.098901098901099No Hit
GCAGACGACACACCGAAGAGTGCTAAGTGGTGTAGGAGCCTCTGGAGAGA11.098901098901099No Hit
ATGGAGGACTGAGGATAGAATTGTTTCACACAGTAGAAATGCATTCTAGA11.098901098901099No Hit
AGTATATGTAGAGCTGGGATTTTCATCTGGGCTGTCAGATCGCTGGGCTG11.098901098901099No Hit
GTTTTACACCTTTGTTCCCAGGCTCTACTGGGTACACTGGCCACCGAGAG11.098901098901099No Hit
TCAATGACCATCTTAATTCGGAAATTTGGCAACATAACTTCTTCTAGTTC11.098901098901099No Hit
GCATGTTTTCTGTTGTTATGTATAGGTTAGATTGGATAGTTTCCTAACCT11.098901098901099No Hit
AAAACAGTGTTCTTAAGCAAAACACATAGTAGAACTAAAACAACTCCAAG11.098901098901099No Hit
GCCTCTGGAGTCATATAGACCTATGTTCAAAACCAAACACCACCATTTAT11.098901098901099No Hit
CCATCCCAGGACTCTGATATCATGACCTGAGCCGAAGGCAGAGGCTAACC11.098901098901099No Hit
CATGACAAGAACCCAGTAAATACTGGCTGAATCGATGAATAAATGTCTAT11.098901098901099No Hit
GTCAGTTATGGTCCATTTTGGTCTGAACACCTTTGTAAGCCCTACAGTTC11.098901098901099No Hit
CCTTGTGCACCTTTCCAGCGTCTGCTGGTAGACTGTACACAGCCCTCCTG11.098901098901099No Hit
TCACCAGTTTTAACCACAGTCTTTAAGACACAGTGAATGCTCACTAAATA11.098901098901099No Hit
CATATAAGATTTTCACATTATAATGATAGTGCCATTAATTTGTTAATCCT11.098901098901099No Hit
GAAGGGTCCAAGGTTCCTTCTACTTACATATTCTAAGAATCACATAACCA11.098901098901099No Hit
CTCAGAGGAAGGACCAGATACTCCCAACACATGAGGGCTTCAGAATTATT11.098901098901099No Hit
GGCTTCCCACGGATCAGGGAGCCCGGTGCTGGGCTCCATCCCAGGTCCCT11.098901098901099No Hit
CATTTTTTGTGTCCTGAAGAAGGCAAATCACCCACTTATGCTAACACCAG11.098901098901099No Hit
ACTCCGCATTGCTTGGCCGCTTTCACTTCCCTGTCCCTTTTCCTGAACTT11.098901098901099No Hit
ATTAAGAACCCCAGAATTCTCACAAGATTTGGCCAAACTCCCCTACACTC11.098901098901099No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG11.098901098901099No Hit
GCAGTACACACTTGGGTTACGACATTAGCTTATGTTAGAGTTGGCGTGTC11.098901098901099No Hit
GTGTAATTGTTCATAGTACATTCTTAAGAGCCTTTGCATATCAATGGTTT11.098901098901099No Hit
GTGTGGGGACCAGCCAGGCGGCAGAAGCACAGGTCGGGGTGGCAGTGATT11.098901098901099No Hit
GATCATACATGGTGCCATATACTGTCAAGAGAAATGTGAACAACTGTAAA11.098901098901099No Hit
GCCATTGCCTCCTCTGCCATTTGGCCATTTTCAGGTTTAGGATGTTGCCC11.098901098901099No Hit
TAGATATCCAAAACGGGCTGAGGATACTTTTTGAAACCTATTAACTTTGC11.098901098901099No Hit
CCACCCAGGCAGCCGGAGACAATCCAGCCTCCCTCTGCTCCAGGGCAAAG11.098901098901099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACC50.0145.01
CTTAGGT50.0145.07
CCACCTT50.0145.03
CACCTTA50.0145.04
TCCACCT50.0145.02
TTAGGTC50.0145.08
ACCTTAG50.0145.05
CCTTAGG50.0145.06
ACTGGAA50.0145.0145
TAGGTCA50.0145.09
Produced by FastQC (version 0.11.5)