FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1795_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868548
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19000.039.3082431
AGATCGT7800.034.395496
AAGATCG8650.030.1773285
GCGAAAG24900.029.994242
CGAAAGC27600.027.5854593
TATACGG3255.0931703E-1126.7727075
TATACTG22500.026.7479155
ATATACG4251.8189894E-1223.8854544
ATCGTGC12300.022.3980968
CCGTAAG8750.021.5459331
TATCGTC1700.003094138821.3238167
GTAATAT22850.021.261241
TATATCG3157.2837975E-720.7169725
CGTAAGA7400.020.577262
GTGATCG9600.018.8802199
TCTCGTT8450.018.875505145
TAATATA28800.018.379362
ATATAAG23100.018.2060621
GTATATA14200.017.8722691
TCTATAC46000.017.8123253
Produced by FastQC (version 0.11.5)