FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1795_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAAATCCCACTTCCTTTTTCTGGGTGTACCTTATTGTTGACTCATTA23.7735849056603774No Hit
TAAATAGACAGACACACAAAGAACCTTATGCCTCCCACTTAAAAATTTTA11.8867924528301887No Hit
GCCCTTCATTGGCTCTTGTTACAGAGTTTGGATTTTTTCTTGTTCTGCTC11.8867924528301887No Hit
CAGCCCGCCTGCGGCCAGTGCCTCCGATACGAGACACAATAATAATTTGA11.8867924528301887No Hit
GGCCATGGCTGGCCTTCGTGCCGCCCTTATAAATGTGAAATGTGTGCTCT11.8867924528301887No Hit
CCCCAGAGACGTAACTAAGACAAGATGACTGGAACTATAAGGGAAGACAC11.8867924528301887No Hit
CCTGGAGAGAGTTTAAATATGCAATCAAACACTTCCACCCAGCATAAGTC11.8867924528301887No Hit
CTGTCGCCTCAGGGAAGAAGAGCCTCGGGAGAGCTGGTGCCTCCTGGGCC11.8867924528301887No Hit
GCATGCACCAGCCGCTCTGGGATCCAAATTGGAGCCTCCTTTTCCTGTGG11.8867924528301887No Hit
GACAATAGGTACTGAGCTCTTAGAGCAGACCAGGATTTCCCATGAATATG11.8867924528301887No Hit
CATTATGGGTTTTCAAAAAATACATCATTAGAGTTTCTTTTCTGAGCATC11.8867924528301887No Hit
TAGCAAAGGTCCTTCTCTTATCACTGAGGAGCATTTGGAACGTCCACAGG11.8867924528301887No Hit
ATTTAATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT11.8867924528301887No Hit
ATGGATACCACACTAGTCAAGCCTACCTCAGGGTGAGCAAGCATGAGAGT11.8867924528301887No Hit
AACCACAGGGAGACACAGAGATCTGTAATGAAGATTCCTGGCTTGCAAGT11.8867924528301887No Hit
GCAGTCCTAGATACACACTAACTTAGAATCATGGATCACAAGATCTAATA11.8867924528301887No Hit
GGCCAGCAGTGAGTGTGGGACCTAAGGTCCAGCCAGATCTCCAGCTAAGG11.8867924528301887No Hit
GATATATTGGCAAGTGTTTGAAAGGGTATGGAAAAGCTAGAACTCCCAAA11.8867924528301887No Hit
GTACATAGGCTTCTCTGGTTACACCTATGCGAGTACCACATAAACAGATC11.8867924528301887No Hit
GATCCAGCATGATGCCTGTGCTTGCCGCTTTACTATCGGTTCTCTGGGCC11.8867924528301887No Hit
CTCCCAGGCAGCCACACAATCACAATGGCCAACAACCGATATAGTGACAA11.8867924528301887No Hit
CACAGGTCGGGGTGACAGTTTTTTGGTAGCGGCGCTATCAAATGCTTTTT11.8867924528301887No Hit
GTGAGAAGATGGAGGCATCTGTCCCAGCTCTGCCACCGAGTATCGCCCGT11.8867924528301887No Hit
CCTTTGTCGTCTCCCTGGGCGGTGGCTGTCTGCTGCTTTGGGAGTAGCTG11.8867924528301887No Hit
GCTGCCCATGCCTTGTTCCCTCTCCTTTTTTAAAAAAAAAAAGATTTTAT11.8867924528301887No Hit
GGGTGGATGTTATTTATATTGTGTGCTTTTTGGAGAACTGTACTGTAAGC11.8867924528301887No Hit
CATGAAAAACACTGGGAACTCCAGGGGTTTAAACTTGAATTTTTGGTACT11.8867924528301887No Hit
ATCCTAAAACAGGAATGAACAAATGCCCAACAATAAAATCCTAAAATGGA11.8867924528301887No Hit
CCTACACTCTCCGCATCCGCGTCCTGAGGTCAGATGAAGGCGCGCGGTCT11.8867924528301887No Hit
TTCTTATGTTGTGGAAATGTCATCACTTGTACAAAGTCCAAGAGGAAAAG11.8867924528301887No Hit
CTTTGAAGCGAAACATAATCATGTGGGGGTGCGGACTGTAGCACTATATG11.8867924528301887No Hit
GTCTGAGAACAACTGAATTGTGGGCTCTCTAAGGACCCTTCCAGATGGAC11.8867924528301887No Hit
CATCCCCGCTACTAGTCTGTAATGGTCTTGTGCACCTGGTTCGGGTTAGA11.8867924528301887No Hit
TAATGGCAGAGGCCGCCCTGGCCAGGGGCCTGCCTCCACCATCTCCCCCT11.8867924528301887No Hit
GATTATTTTTATATGTAACGGTTCTAATACAATCAGTGTGCTAGCATCAT11.8867924528301887No Hit
GGCCGCGGGGATCATGCCCTGAGCCGATGGCAGCGGCTTAAACAACTGCG11.8867924528301887No Hit
GTCTGGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCC11.8867924528301887No Hit
GATACCTACGTCCTCCCTGAGTATGCCAATACTCTAGCAGACCAATCTCC11.8867924528301887No Hit
GTCCCTAGACCTAAATCCCGAGTCTTGGCAGTGGGTACGAACCCAGCTTT11.8867924528301887No Hit
CAGTCAGAGCGTACAGGTGAGTCATGCTCAGCATAAGCACTGCTATGAAG11.8867924528301887No Hit
GAGTTGTAGTGTTGTGACAGAGGCTGTATTGCCTTCAAAAATTGAAATAT11.8867924528301887No Hit
CCCCACGCATCCATTCCCTCTACCTGCAGAACAACTTCATAACGGAGCTC11.8867924528301887No Hit
AGGTGCAGCCTTGCTTTCACCTGGCTAGCTTGCCCGGCCCGGGAGCTCTG11.8867924528301887No Hit
AAGTTGGATTTATTCATTCAGTTAGTCACAAAATGATTTTACCTAAGTGC11.8867924528301887No Hit
GTCGTGAGATATAACCAGCTATGTGTAAAATAACTCAATATCCGATTGGA11.8867924528301887No Hit
TGTGGAAAGTATTACAACACAGCTGACTGCCATGCCAGTTTGGTTGGTTG11.8867924528301887No Hit
GTATAGTGCCTGGGATGGGCATGGAGACATGGAAGGGTTTGGGTGTAGGA11.8867924528301887No Hit
GTTTTAAGCAAAGCCATCGGATTTGCTGATTGACAATAGACACTGCGTCA11.8867924528301887No Hit
GGGTGAGAGTGAGCGGGCTGAGGGAAGAGAGGAAAGACTGAAGAGCACAG11.8867924528301887No Hit
GTAGCGTTAATTTCAATAGTTGTTGAGTGTTCCTCCAAGTTATCTTAGCT11.8867924528301887No Hit
CGTCACTAGGAGACCGTGAGGACAGTGCTGGGGATGTCTCCTGACTAACA11.8867924528301887No Hit
GTTCCTGGGAGAAGAGAGCAGGGCAGACATCATGGAGGAGGTGTGCTTGG11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCT50.0145.05
GTACATA50.0145.01
TACATAG50.0145.02
ACATAGG50.0145.03
AGGCTTC50.0145.07
GGCTTCT50.0145.08
CATAGGC50.0145.04
TCTTTTT50.0145.0145
GCTTCTC50.0145.09
TAGGCTT50.0145.06
Produced by FastQC (version 0.11.5)