FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1795_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4907750
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC74550.1519026030258265No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC64380.13118027609393307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG5350.042.008599
CGAAAGC22800.032.756613
TATACTG28500.030.0215935
GCGAAAG27750.026.3909472
AGCGAAA29100.025.1666241
GTAATAT20950.024.5737191
CGTACGG1650.002600257821.972665
TCTATAC62950.021.3094653
CTATACG2806.0911498E-620.7170814
TATAATA39450.019.6667822
GTCCTAT48700.019.3557911
CGAGACG2256.873749E-419.332985145
GTATAAG13450.018.8687151
GTTCTAT66550.018.3044971
ACGTACG2000.00791421318.1274434
CAATTCG5209.1858965E-1018.1246749
CTATACC65100.017.9325264
TAATACA59050.017.9279044
TATACCA68700.017.7316475
GGTAGTG24800.017.541267
Produced by FastQC (version 0.11.5)