FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1795_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463052
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC76590.17160902449713783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC46850.035.134263
GCGAAAG19850.032.8769572
GTTCTAT50550.031.1277831
GTGATCG4650.029.6242269
AGCGAAA22350.029.1994461
CTATACC58200.028.0329744
ACCGTAC4750.027.4739048
CGAAAGC24550.025.696993
TATACCA67050.025.3064695
TTCTATA67750.025.1517492
CACCGTA5550.024.8208547
TATACTG28050.024.3001795
ATACCAC62350.023.8414066
TACCACT61050.023.6332537
GTCCTAT39400.020.9806181
TAGTACG3351.3144199E-619.4808244
ACCGTCG3751.8115134E-719.3334888
ACCACTG78750.019.0572978
TACACCG11350.018.527495
CTAATAC30650.018.4534933
Produced by FastQC (version 0.11.5)