FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1795_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444219
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC191030.1829050118539261No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT106530.10199901017012378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG10000.047.124218145
GTGATCG16400.031.830729
GCGAAAG50300.024.7959442
TCTATAC157450.024.50133
CGAAAGC51400.024.2652933
AGCGAAA51850.023.355431
GTTCTAT148500.022.950471
GTCCTAT96450.021.878171
TATACTG57350.021.2421325
GTATATA51150.021.1232551
GTATAGG26400.020.3258151
CTATACC173600.020.0081064
CTAGGGT45150.019.9151764
TATACCA187650.019.708065
CGCACTA4102.172601E-819.4511768
GTTGACA66550.019.2861441
TTCTATA188200.018.879772
TACCACT178250.018.0190547
CTAATAC63150.017.9131263
GTTACGT14650.017.819071
Produced by FastQC (version 0.11.5)