FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n01_1795_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1795_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62369
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC670.10742516314194551No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGGG100.0070825294144.948687
ACTGGGC451.1872106E-464.421638
CCTGTAC501.9990894E-457.979473
TGGCACC400.006013718454.39938145
TAGAGTG400.006032857654.355765
GTCCTAC604.917829E-448.3162271
AGGAGTG450.00961055648.3162237
TCCGGAG200.00607387328.994387135-139
TACCACT1000.006041904428.9897354
AGGACCT1000.006041904428.9897355
TTCCAGG1358.014135E-426.8423481
CCCTAAA1100.00962011726.3543031
TCCAGGA2101.5291034E-524.1581132
AGCAGGG2050.00905194817.6766682
GAGAGAG4553.7981514E-412.7427417
AGAGAGA5059.130095E-411.4810848
GGGGGGG22800.06.99875145
Produced by FastQC (version 0.11.5)