FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n01_1794eye_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n01_1794eye_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4362665
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG16700.035.1698342
GTGATCG7950.030.093939
AGCGAAA19600.029.5961821
CGAAAGC21700.027.4003353
TATACTG36850.025.5807115
TACACCG6850.021.1712345
CGTAATA6950.020.8663712
TAGTACT20250.020.7687194
TACATCG10950.020.5246145
ATGCCGG10550.019.241224145
ATACGGC6800.019.1942146
CTAGTAC13750.018.9846043
GTAATAT28600.018.0008931
TATACGG2851.6326261E-417.8098355
TCGCCAT9400.017.738916145
GCGTAAT6553.6379788E-1217.7125231
CGGCATA6202.910383E-1117.5400839
GTATTAT49850.017.4549181
ACTGTGC51550.017.439148
CCACGAC8450.017.159332145
Produced by FastQC (version 0.11.5)